Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How is mathematical giftedness supported in different countries?

I hope for a global overview on mathematical giftedness and its support in school and/or on an extracurricular level. What programmes/opportunities are offered?

08 July 2020 7,124 4 View

GROMACS - how to solve problem coming out during a creation of new Force Field?

Following Gromacs Manual and other suggestions from different topics I create a molecule with Avogadro, I decided to employ oplsaa ff (I copied it in a local directory giving a new name) and I...

25 March 2020 4,438 3 View

How to create a fictitious atom in shelx?

Dear all, I am dealing with some crystal structure in which it is possible to replace Br- anions with Cl- anions. I am trying the refinement by setting the occupancy factor according to the molar...

24 February 2020 4,122 2 View

Trying to run RT-PCR for PDCD4 gene in human serum, can't get any values. Checked primers and they seem okay, any suggestions?

We are having difficulty detecting PDCD4 in serum. We have ordered an ELISA to attempt this, but have not tried it yet. Afraid something is wrong with serum.

08 January 2018 4,637 3 View

How to check for primer dimers?

I am having problems with high efficiency of my primers and one reason could be the formation of primer dimers. I dont see any big peak on the melt curve, but I dont know if this increased peak is...

19 September 2017 7,270 4 View

Checking RNA integrity for qPCR with electrophoresis but it does not show 28S/18S ~ 2. Should I extract again?

I have run RNA samples in 1% agarose gel but I cannot visually see a ratio 28S/18S ~ 2. What I might be doing wrong? Also, the samples marked with numbers, should I still consider them for qPCR?

12 September 2017 6,958 6 View

How can extract eva-copolymer from a wax blend?

Hello everybody,I must extract Ethylene Vinyl Acetate (EVA) copolymer from a mixture consisting of: EVA / Liquid Paraffin / Microcrystalline Wax. Can anybody give me some advice? Thank you

26 April 2017 8,351 3 View

Is there any phenotype database for Streptomyces?

I'm identifying my Streptomyces isolates at the specie level. I already performed all the phenotype / chemotaxonomic tests, and constructed a phylogenetic tree. However, I didn't find the...

16 April 2017 1,713 3 View

Griess Reagent usage in fibroblast cell culture?

Hello, I am trying to measure NO in cell culture (fibroblast cells). Using Griess reagent I try to follow the paper from Wang et al., 1996 which gives a basic protocol that does not differ from...

07 October 2016 8,597 1 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View