Simone Tamburri

5 Questions 16 Answers 0 Followers

Questions related from Simone Tamburri

Crosslinking mass spectrometry to define complex topology. Which tag?

Hi all, I'm looking for suggestions about performing immunoprecipitation of protein complexes in cells, followed by crosslinking todefine the topology of specific complexes. Which tags and which...

08 May 2020 1,182 0 View

What program do I have to use for Daudi transfection using AMAXA?

Hi guys, could you share with me your protocol to transfect DAUDI cells by AMAXA? I have to do siRNA transfection. I have the nucleofector solution V thanks

07 December 2014 6,590 1 View

How is it possible to eliminate polymers?

Recently I observed polymers in my samples. Do you have some suggestions to avoid their presence?

31 May 2014 2,926 7 View

Can anyone give me suggestions on doing nuclear/citoplasm fractionation?

Unfortunately when I performed N/C fractionation I assessed protein precipitation. Usually I used a hypotonic buffer to separate C from the N. On the nuclei I applied a strong saline buffer. The...

06 April 2014 429 4 View

Any suggestions for the fractionation of suspension cells?

I'm having a lot of problems performing nucleus/cyto fractionation in DAUDI cell line. The protein recovery is really low. Do you have any suggestions?

26 March 2014 5,276 3 View