I red a paper on an important journal of cardiology. In this work they found that 8 miRNAs were dysregulated in plasma samples of a study cohor of 1710 participants (Controls + patientes affected by Heart failure). I evaluated these 8 miRNAs in plasma samples in a study-cohort of 129 subjects (Controls + Heart failure patients) and I found that only 2 miRNAs were dysregulated according to the work I red. Because I have to explain thi data in "Discussion" I don't know how to explain this difference. Why could be the main reason of these different results? Maybe the size samples or other? Thank you
Did you the the exact same thing they did in terms of sampling population and methods (reference gene, primers etc). What was their effect sizes, sample statistics such as P-values?
The number of participants and their demographics could be a factor. Race and differences in cardiometabolic issues might be influencing the differences miRNA expression. Try look at the DEGs associated with the miRNAs that didn't match up, this will give you more insight into what's happening. It could be technical differences like choice of housekeeping genes, normalization process.
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