What other methods are there to precipitate phages after enrichment form sewage samples?

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is the box plot of climatic indices better illustrate from daily data or annual data?

Hello, I hope you have a good time. I work on a research project about temperature indices. Due to the high number of indices, I only work on tables and maps on an annual time scale. In other...

19 February 2021 9,742 2 View

How to avoid ATP degradation while performing ATP assay?

I am using the ATP Assay Kit (Colorimetric/Fluorometric) (ab83355) for measuring intracellular ATP from treated cell lines. Although my standards are wokring fine when using the fluorometric...

15 February 2021 3,746 4 View

Should the AUC in a concentration-time graph of a subcutaneous implant be roughly the same for poeple?

I have 2 concentration time curves of a subcut drug implant. the first group has a peak around the 50 day mark where as the second group does not. Both groups received the same implant by the same...

10 February 2021 2,305 3 View

Stats to compare recharge of groundwater and determine if precipitation has more of an effect on groundwater levels before or after resto??

Hello all, I am looking for ways to compare recharge of groundwater and determine if precipitation has more of an effect on groundwater levels before or after restoration? My data set contains a...

08 February 2021 7,887 5 View

What recipe of homemade lenti-virus concentrator do you use?

My lab is using a lenti-X concentrator. And we are looking for a homemade recipe. I have tried this recipe....

08 February 2021 5,965 1 View

Which is the most ecofriendly and effective activated agent for the preparation of activated carbon from sewage sludge?

I am going to prepare activated carbon from the waste activated sludge coming out of WWTP. Can you please help me in understanding, which chemical/physical activating agent will be most...

07 February 2021 9,507 5 View

Separation of CaO from Al2O3 and SiO2 in a solid waste, does HCl work?

I have an alkaline solid waste which contains the following components: SiO2, Al2O3, and CaO according to the XRF analysis (as it gives the results as oxides). However, I know from the XRD that it...

28 January 2021 9,469 3 View

How to get rid of the solvents from a PU-Ag nanocomposite?

I've synthesized a polyurethane-Ag nanocomposite and the product is dissolved in a two-component system i.e. DMF and DMSO. I wish to know of any ways that I can use to get rid of the solvents and...

27 January 2021 1,847 3 View

How can you adjust pH of SM buffer?

Good afternoon, please how can you adjust pH of phage SM buffer? I presume that main ingredient that has influence of pH is Tris/HCl buffer, but according to literature its range of pH is from 7...

27 January 2021 5,767 3 View

Binyamin Kerman

It may be possible to use tangential flow filtration but you would need the necessary equipment. https://www.nature.com/articles/nprot.2009.10

There's also a method for isoelectric precipitation and other flocculation protocols out there. Have a look at these as a starting point. https://link.springer.com/article/10.1007%2Fs00253-013-5049-9 https://www.biorxiv.org/content/10.1101/2020.10.26.356501v1.full

Good luck!

Bhuvesh Khulputeea

Thank you for the reply Binyamin Kerman . But, due to the lack of equipment, we need to focus on the chemical precipitation of the phage. So, I was looking for something with PEG 6000 at first. But, any other chemical methods can also be helpful.

Helena Santiago Lima

Hi Bhuvesh, I recommend that you read this article, because in it, three viral concentration methodologies are tested.

Viral concentration methods for diversity studies in soil samples. DOI:10.1016/j.apsoil.2020.103666

Roberto Dias