Hi All:

I need to precipitate extracellular protein from supernatant.

A bit confused with ammonium sulfate calculation.

Should I be adding:

1. 65g of ammonium sulfate in 100 ml of supernatant?

OR

2. 49.465g of ammonium sulfate in 100 ml?

(According to Mass required for 100 ml=0.65 x 761g/L x 0.1L)

Thanks in advance.

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