Hi All:
I need to precipitate extracellular protein from supernatant.
A bit confused with ammonium sulfate calculation.
Should I be adding:
1. 65g of ammonium sulfate in 100 ml of supernatant?
OR
2. 49.465g of ammonium sulfate in 100 ml?
(According to Mass required for 100 ml=0.65 x 761g/L x 0.1L)
Thanks in advance.