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Questions related from Bhuvesh Khulputeea
I need to obtain pure phage samples from a sample that was enriched using E.coli enrichment in LB. For now, I am using Chloroform, NaCl, PEG 6000 and Phage buffer. Ultracentrifugation is not an...
29 April 2021 4,918 0 View
I cannot find too much detail on it online. I'm lacking PEG 8000 so, i was wondering if I could use these two chemicals instead, but the amount of the chemicals is not known.
12 April 2021 7,699 3 View
I'm in need for protocols for the precipitation of phages (Without need for ultracentrifugation and alternatives for PEG)
12 April 2021 9,026 3 View
For the moment, I am using LB broth to enrich the E. coli to get a concentrated stock of phage but I would like to know if ever there are other media that are more apt in yielding a high phage stock.
09 April 2021 7,678 2 View
I'm basically working with sewage water and I need to isolate and enrich the E.coli colony in the water for bacteriophage isolation. The problem is that in addition to the bacteria, the fugus...
09 April 2021 8,287 5 View