Bhuvesh Khulputeea

5 Questions 5 Answers 0 Followers

Questions related from Bhuvesh Khulputeea

What are the method of purification of enriched phage sample from E.coli enrichment?

I need to obtain pure phage samples from a sample that was enriched using E.coli enrichment in LB. For now, I am using Chloroform, NaCl, PEG 6000 and Phage buffer. Ultracentrifugation is not an...

29 April 2021 4,918 0 View

Is there a method to precipitate phages with PEG 6000 and Dextran sulphate?

I cannot find too much detail on it online. I'm lacking PEG 8000 so, i was wondering if I could use these two chemicals instead, but the amount of the chemicals is not known.

12 April 2021 7,699 3 View

What other methods are there to precipitate phages after enrichment form sewage samples?

I'm in need for protocols for the precipitation of phages (Without need for ultracentrifugation and alternatives for PEG)

12 April 2021 9,026 3 View

What media would you require to get a high yield of phages from E. coli cultures from sewage water?

For the moment, I am using LB broth to enrich the E. coli to get a concentrated stock of phage but I would like to know if ever there are other media that are more apt in yielding a high phage stock.

09 April 2021 7,678 2 View

Is there any method to enrich sewage water bacteria without the fungus present in it?

I'm basically working with sewage water and I need to isolate and enrich the E.coli colony in the water for bacteriophage isolation. The problem is that in addition to the bacteria, the fugus...

09 April 2021 8,287 5 View