Binyamin Kerman

4 Questions 19 Answers 0 Followers

Questions related from Binyamin Kerman

Recommendations for PSMA expressing cell line?

We have been using PC-3 and LNCaP cell lines for negative and positive PSMA expression controls. The LNCaP cells are not the ideal because they grow slowly and don't form an adherent monolayer....

09 June 2022 9,339 2 View

Can antibodies to phage lambda can be purchased?

Does anyone know of a source to find antibodies to bacteriophage lambda? An anti-gpD antibody for the capsid protein would be especially helpful. I haven't seen anything when I search online but...

30 November 2020 3,888 2 View

Any ideas what is going wrong with my bacteriophage plaque assays?

I've been regularly doing plaque assays for a few months using lambda phage to establish the phage titer. It took some time to get the assay to work, at first we didn't dilute sufficiently so we...

26 August 2020 2,091 6 View

Can chloroform be used to remove residual Triton X-114 from recombinant proteins after endotoxin removal?

Does anyone know whether a method exists where chloroform can be added to a recombinant protein sample after performing endotoxin clean-up to remove residual detergents? Our lab lacks access to a...

21 July 2020 6,439 3 View