11 Questions 26 Answers 0 Followers
Questions related from Binyamin Kerman
I am a bit confused whether phosphorylated DNA is needed to achieve efficient homologous recombination in e coli. I believe there are methods that use ssDNA oligos or PCR products as inserts for...
05 January 2024 2,142 1 View
I am attempting to design an oligo to modify the lambda genome using ssDNA recombination roughly following the protocol of Thomason LC, Oppenheim AB, Court DL. Modifying bacteriophage lambda with...
17 August 2023 7,627 4 View
E coli strain JM109(DE3) has its genotype listed as endA1 glnV44 thi-1 relA1 gyrA96 recA1 mcrB+ Δ(lac-proAB) e14- [F' traD36 proAB+ lacIq lacZΔM15] hsdR17(rK-mK+) (λDE3). It seems like it should...
22 June 2023 3,372 2 View
We have been using PC-3 and LNCaP cell lines for negative and positive PSMA expression controls. The LNCaP cells are not the ideal because they grow slowly and don't form an adherent monolayer....
09 June 2022 9,352 2 View
If I understand the Lambda genome correctly it seems that some of the sequence of genes isn't the most logical. When the phage enters the lytic cycle its late rightward promoter first transcribes...
24 March 2022 10,000 2 View
I am trying a new protocol to screen colonies for protein expression where a membrane is placed on the plated colonies and lifted. Then the adsorbed cells are lysed and the protein binds to the...
23 February 2022 9,154 2 View
Does anyone know of a source to find antibodies to bacteriophage lambda? An anti-gpD antibody for the capsid protein would be especially helpful. I haven't seen anything when I search online but...
30 November 2020 3,897 2 View
I am looking for mutant strains of lambda phage that are virulent or strictly lytic. I know there are various mutations that achieve this but can't find any available on ATCC. Does anyone work...
10 September 2020 9,631 1 View
I've been regularly doing plaque assays for a few months using lambda phage to establish the phage titer. It took some time to get the assay to work, at first we didn't dilute sufficiently so we...
26 August 2020 2,138 6 View
I have seen a method for calculating the virion count in phage based on a sample's A269 and A320 values. http://abdesignlabs.com/technical-resources/bacteriophage-spectrophotometry/ This is...
11 August 2020 9,632 4 View
Does anyone know whether a method exists where chloroform can be added to a recombinant protein sample after performing endotoxin clean-up to remove residual detergents? Our lab lacks access to a...
21 July 2020 6,455 3 View
