I want to use the method (T lymphocyte telomere length assay) which has a higher accuracy and validity compared to other methods and in addition to these features, It is allocated the minimum time to measure T lymphocyte telomere length compared to other methods.
Considering what mentioned above, could you tell me what method is better?
Ioakim Spyridopoulos
Multicolour Flow FISH with surface CD3+ antibody. (Obviously you need to test the perfect fluorochrome that is heat resistant, e.g. Q-Dots) Sorting T-cells by either FACS or beads (the latter has better yield, does not require Flow Core facility), and then measuring telomere length by either Southern Blot (if you have enough cells), PCR, or again, Flow-FISH
I think it is difficult to say what is "better" - that will depend on many parameters, such as accuracy required, costs, lab environment, etc... Generally there are 2 possibilities:
1 votes
1 thanks
Mahmoud Delphan
Dear Spyridopoulos
Thank you for your useful explanation. I must study more mentioned methods.
0 votes
0 thanks
Ishak Ozel Tekin
I agree with Ioakim.
My recommendation is double staining by CD3 and classical Flow fish (DAKO).
0 votes
0 thanks
- Badges
- Science method
More Mahmoud Delphan's questions See All
Similar questions and discussions