Why diluted HCl is being used by researchers while extracting free amino acids? When I tried extracting my plant sample with distilled water there was no difference in the extracted amino acids profile.
Dear G. Balaji ,
Amino acid is like Zwitter ion know, so that extracting time should need to maintain pH7 for this purpose Dilute HCL used as a buffer to maintain pH.
You may succeed with water because your system may be almost neutral but in all the case it will be slightly basic because of extraction solvent or nature.,
Hope you got..,
Good Luck & Regards
R.Selva......
By using water, anything thata is water soluble will be extracted, including water soluble proteins, so that if in the subsequent protocol you do not get rid of the proteins, they will be in the final extract injected to the GC or HPLC. By using diluted acids (usually sulfosalicylic acid), most proteins will be precipitated and can be easily removed by centrifugation and/or simple filtration.
Moreover, the solubility of some amino acids in water is rather low, and using a water extraction procedure could lead to underquantifying these amino acids.
To alter pH need to add dil;HCL because of the nature of the system, need to maintain amino acids zwitter ion pH 7 so that.,
Dear sir
We are manufacturing Amino acids and protein hydrolysate based bio stimulant liquid for agriculture .
Both products getting time to dissolve in water . For proper solubility we use KOH , but due to KOH water ph getting high. please suggest Can we use HCL for reduce ph level of solution. Please
- Badges
- Science method