20 Questions 26 Answers 0 Followers
Questions related from G. Balaji
Has anyone isolated and characterized simple straight chain fatty acid sugar esters from plants? Kindly share your experiences.
13 May 2017 1,441 1 View
Both 1H and C13 NMR values of my sample can be assigned to a known molecule except at one place. From reference values and theoretical point of view, a methine proton (CH) near to a methylene...
08 November 2016 8,111 5 View
The only reagent that I could use to see something is Blood reagent but the handling of this reagent is not as easy as anyother reagents. The fraction of such interest has been tried with spray...
01 August 2016 3,254 3 View
I recently came by an article where toluene-ethanol mixture was used in soxhlet extraction. How efficient is this mixture of solvents than using single solvent for extraction?
08 June 2015 3,709 8 View
I hardly find a paper which involves free amino acid estimation. Instead what I get is total amino acid estimation using hydrolysis procedure. So I doubt there are free amino acids present in...
12 March 2015 5,514 8 View
Why diluted HCl is being used by researchers while extracting free amino acids? When I tried extracting my plant sample with distilled water there was no difference in the extracted amino acids...
20 December 2014 1,077 4 View
It would be very helpful for my work if anyone can suggest a way to remove perchloric acid from my sample for further chromatographic studies.
15 November 2014 3,718 3 View
It is intriguing to think about how a potent bio active molecule isolated from marine organism (which must be alien to animal viruses) against HIV and thus involving in human healthcare. How could...
28 September 2014 4,207 4 View
What will happen to the yeasts if the vessel carrying out yeast fermentation is tightly sealed? Will the rising level of CO2 inside the vessel be toxic to the growing yeasts or the yeasts have a...
18 July 2014 3,571 7 View
I am estimating free and bound phenolics (after alkaline hydrolysis) using folin ciocalteu method. No problem with bound phenolics estimation as it gives blue color after incubation and the color...
18 July 2014 7,519 2 View
I have an alkaloid purified from plant origin but it is oily and very difficult to work with. I wonder whether making it to salt form would be better for my further work. Kindly share your...
03 July 2014 4,732 4 View
Can someone suggest a way to solve this problem? Is it a normal phase or a reverse phase the problem with sugar interference exists. My compound of interest in the extract is a water soluble one...
12 June 2014 6,393 3 View
Bound phenolic acids present in fruit requires extreme conditions in vitro to release them from a complex matrix. How does human physiology release and make them available for biological functions?
05 April 2014 6,524 3 View
Initial fractions from Cosmosil column gave a precipitate which dissolves in MeOH or EtOH. But I cant find any spot on the TLC plate via Anisaldehyde-Sulfuric acid or Iodine vapour or Ceric...
01 November 2013 4,545 14 View
How far the use of diethylamine can be allowed or possible in purification of alkaloids by chromatography methods? Does diethylamine at any point of time react with alkaloids during purification...
15 July 2013 7,102 8 View
I like to document the elements present in my fruit sample. Came to know about this EDX spectroscopy and hope it will serve my purpose. My question is whether a single run itself will give a...
19 June 2013 5,179 2 View
Got a water soluble fraction which is white and amorphous but no TLC detection by any means. So it is possible for an organic compound being low. Tried dissolving with acids and it dissolves well...
11 June 2013 1,032 7 View
Can anyone suggest a silica gel (name a brand) exclusively for doing preparative TLC? I am facing additional spots after scraping-off the compound interest from the plates. Possibly due to...
07 February 2013 3,383 7 View
It seems my compound of interest tightly bound to the Alumina column. Most of my impurities in the fraction had been eluted out but still no elution of my compound of interest even after 30%...
15 December 2012 7,223 2 View
I would like to know where in India it is possible to do enantiomeric analysis either by GC or other means. Can anyone give me a suggestion?
27 November 2012 3,399 0 View
