Saima Shakil Malik

5 Questions 26 Answers 0 Followers

Questions related from Saima Shakil Malik

How to optimize a primer, giving same single band at a wide range of temperatures?

We are getting same single band for multiple primers at a wide range of temperatures (50 - 60 °C ) so unable to understand what should be the optimal melting temperature which can be used to...

07 July 2018 7,949 7 View

What is the difference between OS and PFS?

What is the difference between overall survival (OS) and progression free survival (PFS) against any disease?

07 July 2018 6,726 8 View

How to design single pair of primer for fusion genes?

Anyone can help me out to design primers for fusion genes which can be multiplexed later on, for Real time PCR? http://jmd.amjpathol.org/article/S1525-1578(13)00095-0/pdf I am interested to follow...

03 March 2017 225 0 View

Anyone can help me to design primers for ARMS PCR?

How the inner and outer primers can be designed... any software .. ?

05 May 2016 4,113 2 View

Is there anyone worked with Multiplex ARMS-PCR? I am interested to know the procedure...?

http://www.ithanet.eu/ithapedia/index.php/Protocol:Amplification_refractory_mutation_system_(ARMS)

05 May 2016 3,869 4 View