suggest me with refrences
My aim is to create a standard for qPCR for absolute quantification of DNA extracted from soil. The reaction contains PowerUp SYBR Green Master Mix, BSA, primer pair (A189F-MB661R- 0.67 uM), and...
10 June 2024 6,708 9 View
I used miltenyi whole blood CD3 micro beads to separate the T cells from whole human blood using the automacs seperator. Usually after the separation is done by the machine, there is a clear T...
21 February 2024 1,766 2 View
According to a study by Nick Neuwinger et al. (October 2019) on the impact of underfilling vacuum blood collection tubes on lactate dehydrogenase activity, systematic approaches were used to...
20 February 2024 2,166 1 View
I was running PCR 2 lines of fax1 potential mutation (with positive and negative controls). However in the results I have not been able to detect any DNA bands in the gel electrophoresis with one...
31 January 2024 179 3 View
Hello, Our lab is struggling with contamination despite taking every precaution necessary to maintain a sterile environment. The images shown are mature iPSC-derived neurons, but the...
03 October 2023 9,110 4 View
As suggested by the formula by Promega's pGEM-T Easy protocol, (ng of vector x kb size of insert)/kb size of vector x insert:vector molar ratio= ng of insert using example given by protocol,...
12 September 2023 5,543 1 View
I sampled the headspace of my incubation gas, and target the CH4 compound. How do I determine its concentration in unit of "umol g dw soil^-1" ? I have plotted the calibration curve and obtained...
10 August 2023 4,537 1 View
We are culturing hESCs using TeSR AOF kit and passaging with Accutase. The cells were in good condition, but one day after passaging the colonies suddenly formed weird shapes and every day there...
08 August 2023 4,485 2 View
Hello, We have a fisherbrand powerbank (cat. FB300Q) that we use for our western blots. During the transfer, we noticed that it would not reach 90 volts and only get up to 77 volts. We thought it...
07 August 2023 9,090 3 View
Many articles are suggesting CASP software to analyze the comets. Please share the details if there is any free access to this software or any other. Thank you for coming by.
01 May 2023 4,312 5 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.
07 August 2024 4,526 4 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
I'm researching light sources and their UV emissions. I'm curious if a 50W, 7500K conventional LED lamp emits any UV light. I found that a 200W tungsten lamp can emit up to 1.5 mW/cm² at 350 nm....
31 July 2024 3,843 7 View
Following curve related to Co0.6Mn0.4Fe2O4 Magnetic nanoparticles with extra phase a-Fe2O3. I will appreciate to help me to interpretation this spectroscopy, especially the blue line.
31 July 2024 9,140 2 View
Hello everyone, I am new to this characterization but watching videos on youtube I have already plotted a Nyquist plot (Z' and -Z'') and already fitted models on my material using Zsim. But now I...
29 July 2024 1,376 0 View
I've attempted to use this method to synthesize copper nanoparticles. Copper nanoparticles can be synthesised using a variety of precursor materials. CuSO4, distilled water, NaOH, and EDTA are...
28 July 2024 8,027 3 View
How can we calculate the percentage of configuration interaction (CI) in the UV output data of the Gaussian program? for example: Excited State 17: Singlet-A 5.1359 eV 241.41 nm...
28 July 2024 9,165 2 View
While I am doing HER, I noticed that nyquist plots of Electrochemical Impedance Spectroscopy (EIS) is not complete. Will it affect for the Linear Sweep Voltametry (LSV) data? However, I can found...
26 July 2024 7,542 4 View
