Jonathan Jonathan

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Questions related from Jonathan Jonathan

Help on Modified Nanopore Sequencing to do Sanger-like sequencing for PCR amplicon from bisulfite converted DNA?

Hi, I'm planning to do sequencing using Nanopore. The main idea is to substitute Sanger Sequencing that usually used to sequence PCR amplicon from BCD (Bisulfite Converted DNA) using nanopore...

24 April 2024 6,691 0 View

QPCR weird amplification curve, what cause this?

I've done a qPCR reaction using Bio-Rad CFX Duet, 10 ul reaction, using EvaGreen dye. as you can see, image 01 is what I think as a good amplification plot. Today I do the qPCR again using a...

17 February 2023 4,307 3 View

Direct Amplification vs RT for virus RNA diagnostic using LAMP, does RT step is significantly affect the sensitivity?

I have a question about the LAMP reaction regarding the need to add Reverse Transcriptase enzyme for virus (RNA) detection. Is the addition of the RT step crucial in the LAMP method from direct...

14 July 2022 1,079 8 View

Codon Optimization for BL21 and Rosetta 2 cells?

I'm trying to optimize the codon in my sequence for E coli BL21 and Rosetta 2 cells, which codon usage table should i use for this strain? Thank you!

04 February 2022 2,992 2 View

Plasmid Transformation success, but plasmid isolation shows nothing?

I bought a synthetic gene from a vendor, the gene is ligated in the pUC57 plasmid (with ampicilin resistance). the pUC57 plasmid have been tested using electrophoresis and it's all good. I'm...

11 January 2022 1,666 18 View

Protocol for Plasmid integration into Escherichia coli genome?

I'm planning to integrate my plasmid (containing few gene, an enzyme, antibiotic resistance gene, GFP, lac operon, and others) into E coli expression strain. is there any full protocol for this?...

22 November 2021 1,353 8 View

Is disulfide bond in protein like insulin and disulfide ligand is the same thing?

I want to express a protein with this information; single chain, tertiary structure around 500 aa, i dont have any information about the protein beside that. - Is disulfide ligand and disulfide...

05 May 2021 1,017 2 View

Software for searching bacterial expression plasmid by restriction sites?

Hi, so i was looking for a expression plasmid with XbaI and HindIII restriction sites for 1908 bp insert. is there any sofrware to search the plasmid by restriction site? i've been looking for the...

09 April 2021 6,924 4 View