What are some good protocols for native outer membrane protein extraction from E. coli?

I am trying to extract any and all outer membrane proteins from a lab strain of e. coli (ATCC 11229) and am looking for the best general protocol to extract outer membrane proteins. All...

25 January 2021 5,736 3 View

Can you do Ponceau staining after loading control for Western Blot?

Hello everyone! I forgot to take a picture of my Ponceau stain prior to starting my first round of primary and secondary antibodies. I'm currently doing my loading control now and was wondering...

25 January 2021 757 3 View

How to construct Scatter plot for KEGG or GO classification visualization?

Can anybody tell me how to construct this scatter plot for KEGG or GO classification like the image I have attached?

27 September 2020 5,359 3 View

Is RUPKATHA Journal on UGC Care List?

I recently presented a paper in the Rupkatha International Online Conference 2020, and I'm all set to submit my final paper for publication. But I just came across a feedback on the page that...

23 August 2020 1,714 7 View

Approximate timeline in months for Systematic Review without meta analysis?

Hello everyone, I along with few of my colleagues are planning to do systematic review on Covid patients with particular sign ,symptoms and clinical implications mentioned in various published...

14 August 2020 8,096 9 View

Any instances of when an antibody will bind better if left at RT for days?

Can anyone make sense of this: an antibody was left out for 7 days on the bench at RT and binds better to a metalloprotein than if the antibody was taken out the day of the experiment?

15 April 2020 6,330 3 View

Is there any "Biostatistics" online course for Animal Science?

Use of proper statistics to analyse research data is the most important thing. Please suggest me some "Practical" biostatistics course which is available online. Thank you.

19 March 2020 1,931 5 View

How can the "r squared" value of regression analysis be interpreted in Nutritional Requirement study?

Researcher use regression analysis (both linear and quadratic) to express how the growth and other relevant parameters correlated with the graded level of nutrient of interest. In a crude way, it...

13 March 2020 7,064 6 View

Why do most researchers use 0.25M sucrose solution for tissue homogenate preparation?

Generally, the 0.25M sucrose solution is the most common organic solution which is used for tissue homogenate preparation. There are many specific buffer solutions which are used for tissue...

28 February 2020 8,470 2 View

How do I calculate solubility of a compound using a turbidity assay?

I'm planning to measure solubility of my compound in PBS buffer by measuring the turbidity of serially diluted solutions in a microplate reader at 600 nm. Can anyone please inform me how to...

23 November 2019 3,333 3 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

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02 March 2021 3,060 3 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View