Hello,
We have a fisherbrand powerbank (cat. FB300Q) that we use for our western blots. During the transfer, we noticed that it would not reach 90 volts and only get up to 77 volts. We thought it might have been the electrodes, so we switched out the cassette and lid, but unfortunately, that didn't resolve the issue. We also thought it might not be cold enough, so we put a fresh ice pack in the chamber, but that didn't work as well. Also, in the past, we used to put our transfers in the cold room (large walk-in 4C) and sometimes the power banks would switch from volts to amps.
Has anyone else ever experienced this during a transfer? Could this problem be mechanical, or something else?
Thanks for all your help!
Hello Jasmine De Mange
I have experienced something similar but with a different unit. What helped me was adjusting the pH of the transfer buffer to 8.3
Personally, I have seen many labs do not usually set the pH, however, I feel it is necessary to set it so that there is enough ionic strength in the buffer and it helps the powerpack reach the suitable voltage. Please try and let me know if it solves your problem.
Best!
