What is the ratio of absent target in MSAP technique?

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Is there a connection between fixed point theory and number theory?

I was wondering if fixed point theorems like Banach's and Brouwer's fixed point theorems are applied anywhere in number theory?

14 April 2023 6,991 4 View

Has someone work on "Tunneling and propping up"?

Hi, this is Ayoob, I have started work on tunneling and propping up and its impact on firm's performance. As it is my thesis-1. To investigate the existence of "Tunneling and Propping up" in...

04 November 2020 2,591 3 View

Please give me suggestion about topic selecton for research thesis-1?

Hi this is Ayoob, doing certification in Chartered accountancy, and MBA in finance and currently doing internship in audit & assurance firm. So, this is my 2nd last semester of MBA and in...

19 October 2020 2,088 30 View

Which one has the higher organized genome, Wild species or their Cultivated counterparts? Why?

In general, I think wild types has a higher organized genome compared with their cultivated counterparts, and this may be related to breeding and domestication practices that highly affected the...

15 March 2018 412 19 View

Why Epigenetic Mechanisms are the key players in installing plant memory ?

Why Epigenetic Mechanisms are the key players in installing plant memory, while the genetic mechanisms comes second? Thanks in advance.

14 March 2018 758 5 View

If scientists got some money, what they will do with it?

07 March 2018 2,566 13 View

What is the most representative state of DNA methylation?

There are four states of DNA methylation in MSAP technique, Unmethylated, Hemimethylated, Internal cytosine methylation and Full methylation or absence of target! From these, which is the...

04 March 2018 8,947 2 View

Can MetaPhor agarose compete with polyacrylamide?

I have a procedure involves the using of polyacrylamide, but I don't have it! Can I use MetaPhor instead? What limits the using of MetaPhor instead of polyacrylamide?

01 March 2018 3,056 2 View

What kind of relationship between DNA methylation level and gene expression?

I investigated the relationship between DNA methylation level and gene expression in different plant species under different affects, and I found it negative in general. I get confused when I read...

01 March 2018 4,242 4 View

Why does plant still activating the mechanisms of stress tolerance even if growth factors are adequately secured?

The plant in the attached image is a wild type! It have been grown in pots and all growth conditions were in educate levels. Why does the plant still show symptoms of stress! What is the role of...

26 February 2018 1,879 6 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Why in 2D image created by discovery studio 2021, the halogens appear as alkyl group?

In my molecule there is Chloro group at 2-position of phenyl ring, but in 2D image it appears as methyl showing no interaction.

28 July 2024 734 0 View

Are the apoptotic cells is positive for γH2AX ?

It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...

28 July 2024 7,983 2 View

Can anyone help me explain my problem?

Hi, Now I am doing a single-base editing by using HiFi Cas 9, guideRNA, single stranded DNA, HDR Enhancer V2 and my tranfection mehtod is lipofection (RNAiMax). Unfortunately, after gene editing,...

26 July 2024 2,136 0 View

Illustra™ MicroSpin™ G-25 columns what it is used for?

Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...

25 July 2024 4,927 3 View