If scientists got some money, what they will do with it?

If scientists got some money, what they will do with it?

07 March 2018 2,235 13 View

Can MetaPhor agarose compete with polyacrylamide?

I have a procedure involves the using of polyacrylamide, but I don't have it! Can I use MetaPhor instead? What limits the using of MetaPhor instead of polyacrylamide?

01 March 2018 2,866 2 View

What kind of relationship between DNA methylation level and gene expression?

I investigated the relationship between DNA methylation level and gene expression in different plant species under different affects, and I found it negative in general. I get confused when I read...

01 March 2018 4,043 4 View

Why does plant still activating the mechanisms of stress tolerance even if growth factors are adequately secured?

The plant in the attached image is a wild type! It have been grown in pots and all growth conditions were in educate levels. Why does the plant still show symptoms of stress! What is the role of...

26 February 2018 1,677 6 View

Is the gene expression of antioxidants in the different ecotypes is comparable?

We have collected different ecotypes of wild plants species, we intend to evaluate their ability to produce antioxidants by measuring the gene expression of such antioxidant, is that make sens?

25 February 2018 6,061 3 View

What is the suitable kit for extracting DNA from seed tissue?

In many occasions I have problems with DNA extraction from seeds tissue, especially wild types seeds.I tried different protocols, but i still have invalid DNA! Did any body experienced an...

23 February 2018 8,183 4 View

How to evaluate the results of the DNA sequencing?

When you receive DNA sequencing results as in the attached image, is it generally possible to say that the results of the upper row are more confident than the results of the lower in the same image?

28 January 2018 1,229 2 View

Which of the SMAP Passive Soil Moisture products are the best one?

The NASA Soil Moisture Active Passive (SMAP) Produce Soil Moisture Product in HH, VV and dual polarization. Which of these products are the best one? Which of these products are suitable in...

20 July 2017 4,860 3 View

How can I convert Satellite Soil Moisture data (m3/m3) into mm?

30 May 2017 1,064 7 View

Is ther anyone use Preston tube to measure bed shear stress in open channel?

15 January 2017 9,938 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

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17 February 2021 4,217 3 View

Which detection method is better for an ELISA-based assay: colorimetric or fluorometric?

Hello, I am planning to perform an ELISA-based assay to quantify the percentage of genomic methylation (5-methylcytosine) in human embryonic stem cells. I've been searching for some kits and I...

16 February 2021 8,389 3 View