Should the size of diameter of the well diffusion be included or to be subtracted from the result in measuring the zone of inhibition?
As the answers above say, the diameter of the well is usually included, but if this is the case, the measurement will never be zero, and the diameter of the well can vary. For these reasons, we always subtract the well diameter from the measurement, but this must be made clear in the Methods section.
Roger Bayston
Conventional not to substract the well diameter any more than the disc diameter. One must execute the method to obtain a consistent well. Suppose folks in their own lab can use any internal protocol they wish but it's tough to measure the radius from well out and how many such are measured? If wells are so irregular, maybe use an Oxford peni cylinder. and measure diameter.
Article Well diffusion for antifungal susceptibility testing
A ruler is used to measure the cleared area around an agar well. The diameter of the well is included in the measurements
The size of the diameter should be removed while measuring the zone of inhibition
@Adrian Hartanto @Wafaa Eltayeb
if the diameter of the well is included, do you have any reference? journals or articles as literature?
Well diameter is included while measuring the zone of inhibition of discs.
What is proper for antibacterial assay by well diffusion, using the spread or pour method for bacterial suspense?
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