Salah Eddine Sadine the paper not english language sir so i don't understand what their research. do you have any paper that related of my question. Thank you
Beacuse we need highest mortality effect of biolarvacide, when we was pressured appropriate annotation larvacide at last instar, we could assume 1-2sd instars most fatality rate.
It depends on your protocol. If your have perfected your protocol taking first or second instar mosquito larvae, it is fine. You can continue with that. However, as a convention efficacy of any larvicidal agent (chemical larvicie/biolarvicide) is assessed on 3/4 instar larvae. However, it is not necessary always