What is the procedure if we want to compare primers with the others primers if someone have already used the same primer for the genes and our study ?

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How to get nucleotide sequence if we don't know about accession no.?

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Gopal Tiwari

Sorry to say but could you simply your ques. as its not clear.

António Maximiano Fernandes

Normaly should open ncbi run blast past the sequence and press blast

Then will see if the primer match for same species

https://blast.ncbi.nlm.nih.gov/Blast.cgi?PAGE_TYPE=BlastSearch

Atif Idrees

in simple my means can we use the primer as if someone have already use for same purpose