Gopal Tiwari

3 Questions 57 Answers 0 Followers

Questions related from Gopal Tiwari

Can I do western blotting directly with deglycosylated protein?

I expressed my GOI in P.pastoris and deglycosylated the expressed protein using a kit and got expected size band. Now I am planning to do a western blot with deglycosylated protein. Therefore, I...

11 November 2015 5,191 7 View

Do I need to purify the protein expressed in P. Pastoris before deglycosylation with PNGase F?

I cloned my gene of interest in Picz alpha A vector and overexpressed it in P.pastoris X33. When I run SDS PAGE or do western blot I get bigger size protein band than expected which might be due...

05 May 2015 1,669 6 View

Does glycosylated protein shows different band size on nitrocellulose and PVDF membrane??

I expressed protein in P.pastoris and performed zymogram (to check enzyme activity in gel) and did western blotting using Nitrocellulose membrane, in both band observed was in same position...

05 May 2015 9,766 4 View