Atif Idrees

6 Questions 14 Answers 0 Followers

Questions related from Atif Idrees

Which method is best top preserve Fruit flies for RNA extraction?

Hi, I want to check the OBP's gene regulation pattern in B. dorsalis after feed them protein bait but due to unavailability of liquid nitrogen I have to wait for liquid nitrogen. I have feed...

02 February 2016 5,594 2 View

What is difference between quantity and graph during determine RNA cocentration with Nano drop?

hy, I am confused about the parameters which we consider during RNA concentration quantity. My first question is that what is range for high quality RNA ( 1.8-2.1 ul ) for q Rt-PCR and i got 1.9...

01 January 2016 3,244 3 View

Why we always take amino acid sequence instead of nucleotide sequence for homology of two closely related species?

if I do a blast search using my amino acid sequence to look for homologous proteins and then I use my nucleotide sequence will I get the same results? From talking to colleagues the answer is no...

01 January 2016 7,340 5 View

Which are the key points to during design a primer?

I want to know that during Design Primers to check the effect of Protein Bait on the Gene Regulations of of Bactrocera dorsalis which key points should kept in mind regarding GC contents,...

12 December 2015 6,866 6 View

What is the procedure if we want to compare primers with the others primers if someone have already used the same primer for the genes and our study ?

what is the procedure if we want to compare primers with the others primers if someone have already used the same primer for the same genes in same insect species and our study different from...

12 December 2015 3,430 3 View

How to get nucleotide sequence if we don't know about accession no.?

Hy, Please Need your valuable Suggestions, I am going to evaluate the effect of Protein bait on gene expression of odorent binding protein family in Bactrocera dorsalis. Someone has already...

12 December 2015 7,099 3 View