Many trails and efforts had been done and underwent to get a vaccine and get ride of mortality rate of population so what is the percentage that expected of effectiveness of SARS CoV2 as the virus has antigenic shift and drift phenomenon.
The future of stem cells in brain repair and TBI???
08 May 2024 8,126 0 View
Hi everyone, I hope you're doing well. I've encountered an interesting phenomenon in my research and would appreciate your insights. When running gels and staining them with Coomassie, I'm...
18 March 2024 3,416 0 View
Greetings to all, I have attempted to detect pERK using western blotting with HEK 293T cells, but I am unable to do so. I overexpressed a gene that caused pERK to be overproduced, and I was...
21 December 2023 7,195 3 View
Dear ICEM CFD users, Could you please help me to solve this problem? I have a inner geometry inside an other. A coarse mesh of such a configuration captures the inner geometry but a refined mesh...
25 September 2023 2,887 0 View
Hello, Tracheid in Cupressaceae and other conifers may exhibit ornamentation on their walls that resemble helical spiral thickness little bit, as result of an angle in Microfibrils. What is the...
22 January 2023 5,334 2 View
is it possible to do mycoplasma contamination test in my Warthon jelly tissues frozen at -80°C ? all what i found n the internet is compatible with cell culture supernatant and nothing about...
30 November 2022 6,320 3 View
I have completed an experiment where I exposed rainbow trout liver cells to copper concentrations of 0.01, 0.1, 1.0 M, along with a negative control. I have recorded the cell viability % at each...
20 March 2021 9,445 7 View
Biodiesels derived from waste vegetable oil transesterification with Ethanol (FAEE) show higher peroxide values than those derived by transesterification with methanol (FAME) prepared under the...
13 February 2021 515 6 View
I need a spring technician or some who know a lot about heavy duty spring to ask him/her a couple of questions in my bachelor thesis. The question will be in direction of choosing the right...
20 December 2020 8,646 0 View
Will it lose a fraction of its expansion power assuming it was insulated in this system? Because I am designing an insulated gas spring. what I want to know if the gas will lose some of its...
07 December 2020 6,363 3 View
Hello! I planned to do an multiplex RNAscope fluorescent assay for cultured cells in a 96 well plate format according to the protocol provided by ACD:...
22 July 2024 3,334 0 View
hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...
16 July 2024 9,044 10 View
Hello, I've been doing free floating IHC using a VAChT antibody with a 1:1k dilution (139 103 - Synaptic Systems). However, there has been a struggle to keep an even stain with my samples of...
06 July 2024 4,521 2 View
I would like to find a short course focused on the development of monoclonal antibodies covering the entire process, from the selection of antigen and carrier, to the purification of the final...
27 June 2024 5,618 1 View
I am trying to quantify the release of Nitric oxide to test the Immunogenicity of my antigen on U937 cells (Monocytes). Based on the literature, I am supposed to convert 1x105 cells/ml to...
27 June 2024 3,131 2 View
In the molecular dynamics simulation analysis part, how to calculate molecular surface area (MolSA) and polar surface area (PSA) in gromacs ?
14 June 2024 1,469 1 View
How can I use EDSV fiber protein antigen for hemagglutination test?
05 June 2024 1,459 0 View
I am planning to do invitro transcription and capping for an mRNA vaccine generation. Can anyone suggest whether it should be done on a bench top or inside a laminar hood?
14 May 2024 186 3 View
Hello everyone, hope you are doing well. I have FFPE brain arteriovenous malformation (AVM) tissues I have been trying to stain with cleaved-caspase-3. I have used the Asp175 (D3E9) CC3 9579 from...
11 May 2024 1,695 2 View
I have two antibodies that are staining well in IHC but not in IF for tissue sections, when using the same concentration and antigen retrieval method for both. I also know the secondary works well.
08 May 2024 3,890 4 View