What is the optimum time for transfection PEI system?

More Godfrey Azwindini Dzhivhuho's questions See All

Do all T cells have HLA-2A?

I am trying to look at the impact of nef on human MHC 1 the antibody seem not to capture anything. I have analysed this through flow cytometry. summary- cells (CEM-rev)are washed, and stained...

03 April 2018 7,869 2 View

How to titrate an antibody for flow cytometry in a homogeneous cell line?

I have Pe-CD4 antibody that I need to titrate, however, the cells that i have are a CD4+ cell line. this is a problem because all my cells are staining CD4+ with no negative cell population, thus...

01 February 2018 8,048 10 View

Does anyone know if there is a way of updating literature if it is outdated in a thesis: (Any resources; website of skills) ?

I have been writing a thesis over time, and I realise that some of my literature might need some update. The challenge is that there is less literature available out there on the topic if they are...

31 December 2016 6,821 1 View

Soultion that dissolves Praziquantel powder,?

I have purchased praziquantel powder for mouse experiments. I always have a problem with dissolving this powder. I used Distilled water, hot and cold, but still no luck. Is there a proper solution...

04 May 2015 7,686 3 View

How do you set cutoffs for cytokine elisa :IL4 IFN-g IL10?

how do you determine the appropriate cutoff for cytokine elisa to differentiate between positive and false positive?

03 April 2015 4,980 6 View

Is pooling of mice spleen for assays like ELISPOt and flow facs acceptable?

I am faced with an experiment that involves a large number of mice that require elispot and flow cytometry to be done on the same day., n=4 x12 groups, stimulated with 10 different peptides. will...

02 March 2015 4,204 3 View

In mice, does schistosoma mansoni eggs injected I.V induce systemic th2 antibody response? or is it only going to be localized in the lungs?

If I were to inject mice with schisto eggs, I.V, will i get the systemic th2 biased response,? to an extent that spleenocytes and serum will manifest it? down-regulated ifn-g and upregulated IL4?

02 March 2015 9,847 3 View

Why would the presence of IgM not differ between vaccinated and unvaccinated groups of mice?

I performed an ab elisa in mice vaccinated with DNA and gp120 candidate HIV vaccine. Screening the levels of IgG, IgG1 IgG2a IgG2b IgM and IgD in serum. I was wondering if the presence of IgM is...

01 February 2015 6,111 5 View

What are the isotype suitable for th1 and th2 in mice infected with schistosoma mansoni?

I am planing to perform an ELISA assay in mice infected with schistosoma mansoni, and I would like to asses the antibody responses thereafter, how do I determine the correct isotype to look for?

01 February 2015 1,238 3 View

In ELISA IgG standards curve, why are my OD giving me a non linear increase but when i include concentration I don't get the sigmoid shape?

I have done dilution series of the total IgG standards 12 times, when I check the od readings from different dilution series (1:2; 1:3; 1:10) they give a sigma shape graph, but when I align with...

01 February 2015 4,005 8 View

How Social Media Affects Your Mental Health ?

How Social Media Affects Your Mental Health

04 August 2024 6,961 3 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

How does social media influence people's dental habits and interest in aesthetic dental treatments?

Hello, Dear friends, I am surveying as part of my thesis research and would greatly appreciate your participation. The study investigates how social media influences dental health behaviors...

01 August 2024 9,349 2 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Issues with Protein Expression in Transfected cells?

Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...

30 July 2024 6,648 1 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

Does anyone have experience electroporating SKOV3 cells with a large plasmid using Neon transfection?

I have been trying to electroporate SKOV3 cells with a large plasmid (11kb) without much success. Any tips?

29 July 2024 3,229 1 View

Could you try using PeptiCloud and see if it's a useful tool for biology research?

PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...

28 July 2024 4,762 2 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

How to maintain the drug concentration in cell culture study for long term?

I am working in the filed of long acting drugs with Nano formulation. I want to study the efficacy of my formulation in 2D cell cultures while this required media change on every two days. In such...

24 July 2024 8,696 0 View