If I were to inject mice with schisto eggs, I.V, will i get the systemic th2 biased response,? to an extent that spleenocytes and serum will manifest it? down-regulated ifn-g and upregulated IL4?
Dear Godfrey,
Maybe you can find the answer to your question in the article below. Unfortunately I did not find an electronic version of it. I hopo it be useful for you.
Best regards
Hudson
Wynn TA, Eltoum I, Cheever AW, Lewis FA, Gause WC, Sher A. Analysis of cytokine mRNA expression during primary granuloma formation induced by eggs of Schistosoma mansoni.J Immunol. 1993 Aug 1;151(3):1430-40.
Dear Godfrey: I agree the paper mentioned by Dr. Pinto is an excellent resource, although I don't know if it has the answer to your specific question. Personally, I'd think the responses you might see could vary, depending 1) where the eggs were injected, which might affect where they ended up; and 2) the strain of mice you were using. If they were injected in the tail vein, they would probably go to the lungs. If a Th2 dominant mouse strain (such as BALB/C) were used, this could "tilt" the response toward Th2 dominance, but using a Th1 dominant strain might show different effects. JP Caruso, PhD, Veterinary Parasitology, 1983, UGA
Dear Godfrey,
The egg-antigen stimulated type 2 response leads to Th2 cell expansion, production of IL-4, IL-5, and IL-13 accompanied by an upregulation in immunoglobulin (Ig) E levels and circulating eosinophils. The peak of this type 2 response corresponds with the maximal cell response against the egg and is closely associated with the magnitude of granulomatous inflammation surrounding the egg. And I hope the paper below maybe useful for you.
http://www.ncbi.nlm.nih.gov/pubmed/23596444
Best regards
Kong Q. M
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