14 Questions 16 Answers 0 Followers
Questions related from Godfrey Azwindini Dzhivhuho
I am trying to look at the impact of nef on human MHC 1 the antibody seem not to capture anything. I have analysed this through flow cytometry. summary- cells (CEM-rev)are washed, and stained...
04 April 2018 7,834 2 View
I have Pe-CD4 antibody that I need to titrate, however, the cells that i have are a CD4+ cell line. this is a problem because all my cells are staining CD4+ with no negative cell population, thus...
02 February 2018 8,005 10 View
I am transfection 293 cells for viral packaging. Will it be a problem if I change media 6 hrs post transfection. Using the PEI system.
10 October 2017 3,341 2 View
I have been writing a thesis over time, and I realise that some of my literature might need some update. The challenge is that there is less literature available out there on the topic if they are...
01 January 2017 6,802 1 View
I have purchased praziquantel powder for mouse experiments. I always have a problem with dissolving this powder. I used Distilled water, hot and cold, but still no luck. Is there a proper solution...
05 May 2015 7,649 3 View
how do you determine the appropriate cutoff for cytokine elisa to differentiate between positive and false positive?
04 April 2015 4,942 6 View
If I were to inject mice with schisto eggs, I.V, will i get the systemic th2 biased response,? to an extent that spleenocytes and serum will manifest it? down-regulated ifn-g and upregulated IL4?
03 March 2015 9,823 3 View
I am faced with an experiment that involves a large number of mice that require elispot and flow cytometry to be done on the same day., n=4 x12 groups, stimulated with 10 different peptides. will...
03 March 2015 4,170 3 View
I am planing to perform an ELISA assay in mice infected with schistosoma mansoni, and I would like to asses the antibody responses thereafter, how do I determine the correct isotype to look for?
02 February 2015 1,207 3 View
I performed an ab elisa in mice vaccinated with DNA and gp120 candidate HIV vaccine. Screening the levels of IgG, IgG1 IgG2a IgG2b IgM and IgD in serum. I was wondering if the presence of IgM is...
02 February 2015 6,081 5 View
I have done dilution series of the total IgG standards 12 times, when I check the od readings from different dilution series (1:2; 1:3; 1:10) they give a sigma shape graph, but when I align with...
02 February 2015 3,983 8 View
I have ran an elisa assay with one side coated with anti igg and one side coated with SEA protein,.,.i put standads in both sides,.but only the side coated with anti igg showed to have working...
12 December 2014 6,375 2 View
I have prepare dilution buffer and incubation buffer, and I wanted to know how long can i store them in the fridge before the next use
11 November 2014 3,135 5 View
I want to create a panel for T cell and B cell flow cytometry, to investigate how infection affects activation of these cells.
11 November 2014 5,016 9 View
