What are the isotype suitable for th1 and th2 in mice infected with schistosoma mansoni?

More Godfrey Azwindini Dzhivhuho's questions See All

Do all T cells have HLA-2A?

I am trying to look at the impact of nef on human MHC 1 the antibody seem not to capture anything. I have analysed this through flow cytometry. summary- cells (CEM-rev)are washed, and stained...

03 April 2018 7,869 2 View

How to titrate an antibody for flow cytometry in a homogeneous cell line?

I have Pe-CD4 antibody that I need to titrate, however, the cells that i have are a CD4+ cell line. this is a problem because all my cells are staining CD4+ with no negative cell population, thus...

01 February 2018 8,048 10 View

What is the optimum time for transfection PEI system?

I am transfection 293 cells for viral packaging. Will it be a problem if I change media 6 hrs post transfection. Using the PEI system.

09 October 2017 3,377 2 View

Does anyone know if there is a way of updating literature if it is outdated in a thesis: (Any resources; website of skills) ?

I have been writing a thesis over time, and I realise that some of my literature might need some update. The challenge is that there is less literature available out there on the topic if they are...

31 December 2016 6,821 1 View

Soultion that dissolves Praziquantel powder,?

I have purchased praziquantel powder for mouse experiments. I always have a problem with dissolving this powder. I used Distilled water, hot and cold, but still no luck. Is there a proper solution...

04 May 2015 7,686 3 View

How do you set cutoffs for cytokine elisa :IL4 IFN-g IL10?

how do you determine the appropriate cutoff for cytokine elisa to differentiate between positive and false positive?

03 April 2015 4,980 6 View

Is pooling of mice spleen for assays like ELISPOt and flow facs acceptable?

I am faced with an experiment that involves a large number of mice that require elispot and flow cytometry to be done on the same day., n=4 x12 groups, stimulated with 10 different peptides. will...

02 March 2015 4,204 3 View

In mice, does schistosoma mansoni eggs injected I.V induce systemic th2 antibody response? or is it only going to be localized in the lungs?

If I were to inject mice with schisto eggs, I.V, will i get the systemic th2 biased response,? to an extent that spleenocytes and serum will manifest it? down-regulated ifn-g and upregulated IL4?

02 March 2015 9,847 3 View

Why would the presence of IgM not differ between vaccinated and unvaccinated groups of mice?

I performed an ab elisa in mice vaccinated with DNA and gp120 candidate HIV vaccine. Screening the levels of IgG, IgG1 IgG2a IgG2b IgM and IgD in serum. I was wondering if the presence of IgM is...

01 February 2015 6,111 5 View

In ELISA IgG standards curve, why are my OD giving me a non linear increase but when i include concentration I don't get the sigmoid shape?

I have done dilution series of the total IgG standards 12 times, when I check the od readings from different dilution series (1:2; 1:3; 1:10) they give a sigma shape graph, but when I align with...

01 February 2015 4,005 8 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View