How to titrate an antibody for flow cytometry in a homogeneous cell line?

I have Pe-CD4 antibody that I need to titrate, however, the cells that i have are a CD4+ cell line. this is a problem because all my cells are staining CD4+ with no negative cell population, thus...

01 February 2018 7,898 10 View

What is the optimum time for transfection PEI system?

I am transfection 293 cells for viral packaging. Will it be a problem if I change media 6 hrs post transfection. Using the PEI system.

09 October 2017 3,280 2 View

Does anyone know if there is a way of updating literature if it is outdated in a thesis: (Any resources; website of skills) ?

I have been writing a thesis over time, and I realise that some of my literature might need some update. The challenge is that there is less literature available out there on the topic if they are...

31 December 2016 6,737 1 View

Soultion that dissolves Praziquantel powder,?

I have purchased praziquantel powder for mouse experiments. I always have a problem with dissolving this powder. I used Distilled water, hot and cold, but still no luck. Is there a proper solution...

04 May 2015 7,574 3 View

How do you set cutoffs for cytokine elisa :IL4 IFN-g IL10?

how do you determine the appropriate cutoff for cytokine elisa to differentiate between positive and false positive?

03 April 2015 4,854 6 View

In mice, does schistosoma mansoni eggs injected I.V induce systemic th2 antibody response? or is it only going to be localized in the lungs?

If I were to inject mice with schisto eggs, I.V, will i get the systemic th2 biased response,? to an extent that spleenocytes and serum will manifest it? down-regulated ifn-g and upregulated IL4?

02 March 2015 9,751 3 View

Is pooling of mice spleen for assays like ELISPOt and flow facs acceptable?

I am faced with an experiment that involves a large number of mice that require elispot and flow cytometry to be done on the same day., n=4 x12 groups, stimulated with 10 different peptides. will...

02 March 2015 4,089 3 View

What are the isotype suitable for th1 and th2 in mice infected with schistosoma mansoni?

01 February 2015 1,133 3 View

Why would the presence of IgM not differ between vaccinated and unvaccinated groups of mice?

I performed an ab elisa in mice vaccinated with DNA and gp120 candidate HIV vaccine. Screening the levels of IgG, IgG1 IgG2a IgG2b IgM and IgD in serum. I was wondering if the presence of IgM is...

01 February 2015 6,022 5 View

In ELISA IgG standards curve, why are my OD giving me a non linear increase but when i include concentration I don't get the sigmoid shape?

I have done dilution series of the total IgG standards 12 times, when I check the od readings from different dilution series (1:2; 1:3; 1:10) they give a sigma shape graph, but when I align with...

01 February 2015 3,916 8 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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03 March 2021 5,360 2 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View