what is the normal range for CYP1B1 enzyme concentration in human serum?
Hi In the assay of total antioxidant capacity using ELISA kit purchased from Mybiosource company /US , at one of the detection steps and after adding HRP-antibody conjugate, I must add two...
07 August 2018 2,305 1 View
Dear colleagues, how can I know the components and the function of the each buffer used in DNA extraction kit based on the adsorption technique? Knowing that the required information are not...
07 August 2018 555 6 View
EC 1.14.14.1 is the accession number of CYP1B1 enzyme. I know that the first number '1' refers to the major class of the enzyme catalysis reaction, I want to understand the meaning of the rest of...
07 August 2018 8,621 9 View
Many researchers tend to register the DNA sequence that they work on in NCBI or DDBJ or EMBL. What is the benefit of the registration for the researcher and for in general and what is the...
06 July 2018 3,018 5 View
Hi Dear colleagues I have received the referees' reports from a journal that i had submit a research article into for publication.the referees asked me some questions in their reports, i wrote...
06 July 2018 8,148 13 View
Dear colleagues, i need a free link to download graphpad prism
06 July 2018 5,099 2 View
using buffers is almost always being demanded in the analytical procedures related to the biological fluids, so what is the general purpose of using buffer solution ?
06 July 2018 3,061 9 View
in the synthesis of TaqI endonuclease using Ecoli strain by NEB company for example, where from they get the gene coded for this enzyme, what is the function of this enzyme in the original...
06 July 2018 4,069 4 View
Enzymes have activity and concentration. which assay is more reliable in case I want to study the role of some enzyme on some clinical case. Is it determining its activity or its concentration...
06 July 2018 8,729 5 View
Hi I want to understand who to extract an enzyme such as a restriction endoneuclease from a bacteria such as E-coli, what is the simplest technique?
06 July 2018 7,192 6 View
I'm interested in learning about enzyme usage. Could someone explain the basics of working with enzymes? I understand they're different from standard chemicals, but I'm uncertain about the...
01 August 2024 5,460 5 View
I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....
31 July 2024 3,756 0 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
Dear All, My lab primarily works on insect wing patterns. In one of the projects, my student and I have defined 19 abnormality characters on the forewing and 6 abnormality characters on the...
31 July 2024 6,464 5 View
I want to choose a resarch topic regarding enzyme inhibition. So I did my research and found out most of the diseases that originate from enzymes were actually caused by the "deficiency" of...
30 July 2024 2,483 5 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
I am developing an ELISA kit for rotavirus A (G1P8 and G1P6). Does anyone know the weight (ng) of these two strains? Or to help me find their weight?
29 July 2024 3,138 2 View
I have two groups that I test on three different tasks. I have 4 independent t-test, 6 paired t-test and 2 ANCOVA. My concern for which t-test should I conduct bonferroni correction. At the moment...
28 July 2024 7,827 6 View
I am working on carbonic anhydrase immobilization into MOFs. I am facing problems with low enzyme loading.. The other issue is that when using p-NPA activity test to detect the activity of the...
20 July 2024 1,440 3 View
Hello, when calculating the p value for the alleles in the table, how do we place the values in the chi-square test in the four-eyed table? Thank you very much for your attention.
17 July 2024 998 2 View