what is the normal range for CYP1B1 enzyme concentration in human serum?
Hi In the assay of total antioxidant capacity using ELISA kit purchased from Mybiosource company /US , at one of the detection steps and after adding HRP-antibody conjugate, I must add two...
07 August 2018 2,167 1 View
Dear colleagues, how can I know the components and the function of the each buffer used in DNA extraction kit based on the adsorption technique? Knowing that the required information are not...
07 August 2018 373 6 View
EC 1.14.14.1 is the accession number of CYP1B1 enzyme. I know that the first number '1' refers to the major class of the enzyme catalysis reaction, I want to understand the meaning of the rest of...
07 August 2018 8,472 9 View
Many researchers tend to register the DNA sequence that they work on in NCBI or DDBJ or EMBL. What is the benefit of the registration for the researcher and for in general and what is the...
06 July 2018 2,877 5 View
Hi Dear colleagues I have received the referees' reports from a journal that i had submit a research article into for publication.the referees asked me some questions in their reports, i wrote...
06 July 2018 7,989 13 View
Dear colleagues, i need a free link to download graphpad prism
06 July 2018 4,788 2 View
using buffers is almost always being demanded in the analytical procedures related to the biological fluids, so what is the general purpose of using buffer solution ?
06 July 2018 2,934 9 View
in the synthesis of TaqI endonuclease using Ecoli strain by NEB company for example, where from they get the gene coded for this enzyme, what is the function of this enzyme in the original...
06 July 2018 3,929 4 View
Enzymes have activity and concentration. which assay is more reliable in case I want to study the role of some enzyme on some clinical case. Is it determining its activity or its concentration...
06 July 2018 8,591 5 View
Hi Dear colleagues, i had registered 3 DNA fragments in NCBI through DDBJ gene bank , i understand that the two gene banks in addition to EMBL gene bank are linked and the data is interchanged...
06 July 2018 5,890 2 View
How to manually calculate p value in ANOVA? what is the equation or formula for it?
03 March 2021 4,928 5 View
Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...
03 March 2021 1,499 2 View
If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis
02 March 2021 7,670 3 View
Pls share your thoughts
01 March 2021 7,330 4 View
I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...
28 February 2021 7,370 3 View
Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...
28 February 2021 7,310 3 View
I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.
28 February 2021 6,512 3 View
Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?
28 February 2021 3,653 4 View
I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.
28 February 2021 5,929 2 View
We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...
28 February 2021 1,149 1 View