Determination of total antioxidant capacity using ELISA kit ?

Hi In the assay of total antioxidant capacity using ELISA kit purchased from Mybiosource company /US , at one of the detection steps and after adding HRP-antibody conjugate, I must add two...

07 August 2018 2,167 1 View

What are the components and the function of the buffers used in DNA extraction kit from Geneaid company?

Dear colleagues, how can I know the components and the function of the each buffer used in DNA extraction kit based on the adsorption technique? Knowing that the required information are not...

07 August 2018 373 6 View

What is the meaning of the numbers in the enzyme accession number EC1.14.14.1?

EC 1.14.14.1 is the accession number of CYP1B1 enzyme. I know that the first number '1' refers to the major class of the enzyme catalysis reaction, I want to understand the meaning of the rest of...

07 August 2018 8,472 9 View

What is the benefit of registering a DNA sequence in NCBI?

Many researchers tend to register the DNA sequence that they work on in NCBI or DDBJ or EMBL. What is the benefit of the registration for the researcher and for in general and what is the...

06 July 2018 2,877 5 View

Publishing a research article ?

Hi Dear colleagues I have received the referees' reports from a journal that i had submit a research article into for publication.the referees asked me some questions in their reports, i wrote...

06 July 2018 7,989 13 View

Why does using buffers is essential in ELISA procedure and in DNA extraction procedure?

using buffers is almost always being demanded in the analytical procedures related to the biological fluids, so what is the general purpose of using buffer solution ?

06 July 2018 2,934 9 View

How to isolate TaqI endonuclease from E-coli ?

in the synthesis of TaqI endonuclease using Ecoli strain by NEB company for example, where from they get the gene coded for this enzyme, what is the function of this enzyme in the original...

06 July 2018 3,929 4 View

What is the normal range for CYP1B1 enzyme concentration in human serum?

what is the normal range for CYP1B1 enzyme concentration in human serum?

06 July 2018 3,301 0 View

Which assay is more reliable for an enzyme, determining its activity or its concentration and why?

Enzymes have activity and concentration. which assay is more reliable in case I want to study the role of some enzyme on some clinical case. Is it determining its activity or its concentration...

06 July 2018 8,591 5 View

How to register in EMBL gene bank?

Hi Dear colleagues, i had registered 3 DNA fragments in NCBI through DDBJ gene bank , i understand that the two gene banks in addition to EMBL gene bank are linked and the data is interchanged...

06 July 2018 5,890 2 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View