07 July 2018 2 5K Report

Dear colleagues, i need a free link to download graphpad prism

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Determination of total antioxidant capacity using ELISA kit ?

Hi In the assay of total antioxidant capacity using ELISA kit purchased from Mybiosource company /US , at one of the detection steps and after adding HRP-antibody conjugate, I must add two...

07 August 2018 2,305 1 View

What are the components and the function of the buffers used in DNA extraction kit from Geneaid company?

Dear colleagues, how can I know the components and the function of the each buffer used in DNA extraction kit based on the adsorption technique? Knowing that the required information are not...

07 August 2018 555 6 View

What is the meaning of the numbers in the enzyme accession number EC1.14.14.1?

EC 1.14.14.1 is the accession number of CYP1B1 enzyme. I know that the first number '1' refers to the major class of the enzyme catalysis reaction, I want to understand the meaning of the rest of...

07 August 2018 8,621 9 View

What is the benefit of registering a DNA sequence in NCBI?

Many researchers tend to register the DNA sequence that they work on in NCBI or DDBJ or EMBL. What is the benefit of the registration for the researcher and for in general and what is the...

06 July 2018 3,018 5 View

Publishing a research article ?

Hi Dear colleagues I have received the referees' reports from a journal that i had submit a research article into for publication.the referees asked me some questions in their reports, i wrote...

06 July 2018 8,148 13 View

Why does using buffers is essential in ELISA procedure and in DNA extraction procedure?

using buffers is almost always being demanded in the analytical procedures related to the biological fluids, so what is the general purpose of using buffer solution ?

06 July 2018 3,061 9 View

What is the normal range for CYP1B1 enzyme concentration in human serum?

what is the normal range for CYP1B1 enzyme concentration in human serum?

06 July 2018 3,440 0 View

How to isolate TaqI endonuclease from E-coli ?

in the synthesis of TaqI endonuclease using Ecoli strain by NEB company for example, where from they get the gene coded for this enzyme, what is the function of this enzyme in the original...

06 July 2018 4,069 4 View

Which assay is more reliable for an enzyme, determining its activity or its concentration and why?

Enzymes have activity and concentration. which assay is more reliable in case I want to study the role of some enzyme on some clinical case. Is it determining its activity or its concentration...

06 July 2018 8,729 5 View

What is the most general method to isolate an enzyme from a bacteria?

Hi I want to understand who to extract an enzyme such as a restriction endoneuclease from a bacteria such as E-coli, what is the simplest technique?

06 July 2018 7,192 6 View

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Is the mentioned CV graph a valid one as this graph have only one peak prominent (reduction)?

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