In vitro maturation of human oocytes could be an attractive strategy for in vitro fertilization treatment. However, current in vitro maturation protocols unfortunately produce oocytes with a poor clinical outcome. Over the last 30 years, advances in culture conditions have continuously improved IVM's efficiency but even now in-vitro maturation does not support all nuclear/cytoplasmic changes that occur physiologically as a result of ovulatory stimulus in vivo. Adding EGF family molecules (e.g. amphiregulin, epiregulin) to the culture media increases the IVM rate of immature human oocytes. Additionally, brain-derived neurotrophic factor (BDNF) and glial-cell-derived neurotrophic factor (GDNF) also could improve the IVM outcome. Delaying the maturation process by adding cAMP or kinase inhibitors is another option to combine with cytoplasmic maturation. Do you have experience with these supplements? What other options do you apply in your practice?
Indeed, IVM in human oocytes is not successful as in cow (and other species) oocytes.
Do you try IVM in oocytes retrieved in natural or stimulated cycles? Are the oocytes retrieved after HCG (or LH) administration? If this is the case, you should take into consideration that these oocytes have failed to mature. Therefore, the results of IVM are expected not to be the best.
Do you try IVM in cumulus - oocyte complexes (COCs) or in denuded oocytes? You should take into consideration that maturation media have been designed for intact COCs. Therefore, you should have better results with COCs than with denuded oocytes.
Do not forget to add gonadotropins in maturation media.
In any case, to my opinion, maturation media are far from being optimal for IVM of human oocytes. That means, there is plenty of space for experimentation.
Interesting question.
check this link
https://academic.oup.com/humrep/article/26/1/76/705574
Best wishes
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