Smart cities and the role of citizens .What is your opinion about the platforms for civic activity ?

What is your opinion about the platforms for civic activity and their influence on the formation of Public Policies? Can platforms provide a better urban environment?

10 December 2020 9,796 12 View

Can I do RNA electroporation without cleanup after in vitro transcription?

I am working on an EMCV like virus with about 8kbp genome. The RNA is usually prepared from digested plasmids by in-vitro transcription, followed by DNase I digestion, followed by P/C and...

02 November 2020 442 3 View

How would you explain a case with unusually strong expression of p16ink4a in human endometrium?

The endometrial biopsy was taken in the mid-lutheal phase of the menstrual cycle from a woman with recurrent implantation failure (RIF). Extremely high percentage of stromal and luminal epithelial...

11 June 2019 2,583 1 View

Please help, can someone explain me the physical meaning in details for the so called Slater-Condon parameters (integrals) ?

Could you provide me any information (articles) that explains the meaning of the Slater - Condon parameters, and how they can be calculated ?

17 October 2018 6,275 2 View

What is the most useful combination of immunohistochemical/immunofluorescent biomarkers for detection of senescent cells in human tissues?

Identification and quantification of senescent cells in human tissues is a challenging task. It is well known that the application of a single biomarker such as SA-b-gal activity (the most widely...

22 January 2018 9,037 4 View

What is the most appropriate indicator for successful treatment of Lyme disease?

IgM and IgG antibody levels are widely used in the routine practice. However, they may be elevated for years even after successful treatment with antibiotics. What other options ( such as C6...

22 November 2017 1,739 1 View

Covid-19 vaccination in asymptomatic sufferers? Immune response treatment or vaccination?

Covid-19 vaccination in asymptomatic sufferers? Immune response treatment or vaccination?

01 January 1970 5,445 4 View

Bill Gates or the mosquitoes are more dangerous for the planet?

Bill Gates propose to kill al species of mosqitoes on the planet, but they are food for fishes, birds et cetera. The animals (fishes and birds) are healthy irrespectively of the mosquitoes. The...

01 January 1970 4,263 9 View

There is some drugs, which helps for coronavirus infection

There is some drugs, which helps for coronavirus infection - for example chloroquine, tocilizumab, remdesivir, teicoplanin etc. Do you know any other drugs for coronavirus?

01 January 1970 6,969 41 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

No title

02 March 2021 3,060 3 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View