What is the most efficient, least toxic vector for shRNA delivery to the brain?

Argel Aguilar-Valles @Argel_Aguilar-Valles

07 July 2018 1 9K Report

I would like to knock down some proteins in the brain. I have used before siRNAs + JetSI transfection reagents for transient knock downs, but would like to do something longer lasting.

I've seen some publications where some AAVs are shown to be neurotoxic or that they "saturate" the microRNA machinery. Can anybody recommend a good, not-very toxic AAV or other viral vector?

Thank you

Argel Aguilar-Valles

Thank you very much for your answers, they both were really helpful!

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