How to scale up forest carbon stock assessment from plot scale to regional scale?

The research focuses on the mangrove forest ecosystem. Then it will conduct biomass and carbon stock estimation at the plot scale? How to scale up forest carbon stock assessment from plot scale to...

29 December 2020 1,421 3 View

Can I run Structural Equaton Modeling with three independent variables and three dependent variables?

Hello everybody, I have three independent variables and three dependent variables. I want to run the regression analysis with SEM amos. So is it possible to run with the three DVs and three IVs in...

27 November 2020 3,269 6 View

Do I need to code the reverse questions ?

Do I need to code the reverse questions ? In my research, I tested child-centered practices and teacher-centered practices. For example, in child-centered practices, the item is "Teacher has clear...

15 October 2020 2,954 7 View

How the presence of salt affect the water-in-oil Pickering emulsion stability ?

I have prepared the stable water-in-oil Pickering emulsion by using stearic acid-modified CaCO3 nanoparticles. The oil phase is toluene and the dispersed phase is deionized water. I want to use...

21 September 2019 9,938 3 View

Can I use collapse command by village id, year, and ecological zone (hilly, dry, flat)?

Hello, Can I use collapse command by village id, year, and ecological or geographical zone? Is the state command collapse (mean) weight height, by (villageID Year GeographicZone) ? How do I...

13 January 2019 3,653 2 View

Is my Protei Quantitation Assay correct?

I am doing western blot experiment and in protein assay, with BCA reagent, I could not achieve my required loading protein amount. I could achieve only 4 or 5 microgram per microliter. But I could...

03 January 2019 2,277 6 View

How to use SPSS for validity check on pairwise questionnaire?

Hello, experts! I am doing my research by using AHP method. I have 4 main criteria, 14 sub-criteria and 5 alternatives and I have 30 experts. I have already done AHP analysis. But I need to do...

19 October 2018 2,334 3 View

What is the recommended sample size requirement for a questionnaire validation?

What is the recommended sample size for a questionnaire validation for a PhD project?

04 April 2018 3,534 3 View

How would you translate a 96 well optimization for lipofectamine and DNA concentration to a 6 well plate setting?

How would you translate a 96 well optimization for lipofectamine and DNA concentration to a 6 well plate setting? If my results for the 96 well plate is 0.35 ul lipo and 200ng DNA, how would you...

29 January 2018 8,735 3 View

MAPK signaling pathway in cisplatin induced apoptosis Jurkat T-cells?

Dear fellow researchers, I am working on a project to globally determine the phosphorylation quantitative profiles of cisplatin-induced apoptosis in Jurkat T-cells. I have 4 biological replicates...

13 October 2016 2,315 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View