How to scale up forest carbon stock assessment from plot scale to regional scale?

The research focuses on the mangrove forest ecosystem. Then it will conduct biomass and carbon stock estimation at the plot scale? How to scale up forest carbon stock assessment from plot scale to...

29 December 2020 1,421 3 View

Can I run Structural Equaton Modeling with three independent variables and three dependent variables?

Hello everybody, I have three independent variables and three dependent variables. I want to run the regression analysis with SEM amos. So is it possible to run with the three DVs and three IVs in...

27 November 2020 3,269 6 View

Do I need to code the reverse questions ?

Do I need to code the reverse questions ? In my research, I tested child-centered practices and teacher-centered practices. For example, in child-centered practices, the item is "Teacher has clear...

15 October 2020 2,954 7 View

How the presence of salt affect the water-in-oil Pickering emulsion stability ?

I have prepared the stable water-in-oil Pickering emulsion by using stearic acid-modified CaCO3 nanoparticles. The oil phase is toluene and the dispersed phase is deionized water. I want to use...

21 September 2019 9,938 3 View

Can I use collapse command by village id, year, and ecological zone (hilly, dry, flat)?

Hello, Can I use collapse command by village id, year, and ecological or geographical zone? Is the state command collapse (mean) weight height, by (villageID Year GeographicZone) ? How do I...

13 January 2019 3,653 2 View

Is my Protei Quantitation Assay correct?

I am doing western blot experiment and in protein assay, with BCA reagent, I could not achieve my required loading protein amount. I could achieve only 4 or 5 microgram per microliter. But I could...

03 January 2019 2,277 6 View

How to use SPSS for validity check on pairwise questionnaire?

Hello, experts! I am doing my research by using AHP method. I have 4 main criteria, 14 sub-criteria and 5 alternatives and I have 30 experts. I have already done AHP analysis. But I need to do...

19 October 2018 2,334 3 View

What is the recommended sample size requirement for a questionnaire validation?

What is the recommended sample size for a questionnaire validation for a PhD project?

04 April 2018 3,534 3 View

How would you translate a 96 well optimization for lipofectamine and DNA concentration to a 6 well plate setting?

How would you translate a 96 well optimization for lipofectamine and DNA concentration to a 6 well plate setting? If my results for the 96 well plate is 0.35 ul lipo and 200ng DNA, how would you...

29 January 2018 8,735 3 View

MAPK signaling pathway in cisplatin induced apoptosis Jurkat T-cells?

Dear fellow researchers, I am working on a project to globally determine the phosphorylation quantitative profiles of cisplatin-induced apoptosis in Jurkat T-cells. I have 4 biological replicates...

13 October 2016 2,315 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View