Can you offer advice on cloning microRNA in expression vector?

Hi !! Has anyone tried cloning a microRNA in expression plasmid ? i am trying to clone the premature sequence of the microRNA of interest in pCDNA3.1. i use genomic dna as template. i am not able...

06 July 2015 2,812 3 View

How do I amplify a 10 kb fragment via PCR?

Hi ! I need to clone a 10 kb fragment into a GFP vector. To start with the PCR amplification, I am not able to amplify it. The primer Tm are Fw- 52.3 , Rv- 50.8. The GC contents are 55.8% and 45%...

03 April 2015 872 16 View

Can anyone recommend a protocol for GLIOMA animal modelling ?

01 February 2015 6,137 5 View

Hi, I have two enzymes - EcoR1 and Kpn1. Both the enzyme expirations dates in 2010. Can I use these two enzymes for cloning?

Hi, I have two enzymes - EcoR1 and Kpn1. Both the enzyme expirations dates in 2010. Can I use these two enzymes for cloning? Will it work and, if yes, will there be proper restriction overhangs?...

09 October 2014 1,552 14 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

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22 February 2021 9,932 11 View

Is there a method for evaluating nanoparticle adhesion to epithelial tissue in vitro?

I want to measure how well nanoparticles can transfect cells based on NP adhesion properties. In the literature I find people do it in animal studies, and epithelial adhesion helps in transfection...

21 February 2021 7,791 3 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View

How to select therorical or conceptual framework for any proposed qualitative research work?

Hi, I am conducting an exploratory research with qualitative in-depth interview approach to understand the impact of COVID_19 in health systems response to burn care in India. Since, this is a...

19 February 2021 4,562 4 View

How to model rupture in geogrid in abaqus?

Hi researchers I wanna model tensile test on geogrid in abaqus, so I added displacement to one side of geogrid and the other side of geogrid is constrained completely, everything seems normal but...

19 February 2021 2,195 2 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View