What is the maximum microRNA concentration that can be used for in-vitro studies?

More Roshini Arivazhagan's questions See All

Can you offer advice on cloning microRNA in expression vector?

Hi !! Has anyone tried cloning a microRNA in expression plasmid ? i am trying to clone the premature sequence of the microRNA of interest in pCDNA3.1. i use genomic dna as template. i am not able...

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How do I amplify a 10 kb fragment via PCR?

Hi ! I need to clone a 10 kb fragment into a GFP vector. To start with the PCR amplification, I am not able to amplify it. The primer Tm are Fw- 52.3 , Rv- 50.8. The GC contents are 55.8% and 45%...

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Can anyone recommend a protocol for GLIOMA animal modelling ?

Hi Has anyone tried GLIOMA animal modelling ? It will be really helpful if anyone could kindly suggest me a good protocol to follow.

01 February 2015 6,187 5 View

Hi, I have two enzymes - EcoR1 and Kpn1. Both the enzyme expirations dates in 2010. Can I use these two enzymes for cloning?

Hi, I have two enzymes - EcoR1 and Kpn1. Both the enzyme expirations dates in 2010. Can I use these two enzymes for cloning? Will it work and, if yes, will there be proper restriction overhangs?...

09 October 2014 1,613 14 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

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Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

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Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

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How do we pick data for determination of Validation Acceptance Criteria?

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When I am trying to distill triethyl amine while drying it over calcium hydride. How do I design the setup to let hydrogen escape without losing NEt3?

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Does anyone notice the tumor regression impact of Hepa1-6 cells in HCC orthotopic mice model?

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Techniques regarding protein-protein interaction in vitro?

What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?

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How can we identify (in silico) the interacting amino acid residues or the nucleotides involved in the Protein-Protein / Protein-RNA interaction?

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How can i generate a CRISPR knockin mutation zebrafish model with a reporter?

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Trang Nguyen

In the protocol of Santa Cruz, it is recommend to use maximum is 250nM. The minimum concentration is 60nM. I think it will depend on the company and siRNA concentration.

Md Mahmodul Hasan Sohel

Hi Roshini,

I am totally agree with Kamil. You need to mention the cell type, purpose of the experiment and downstream experiments. I was working with granulosa cells and 50nM was working well in my case.

Wish you good luck with your work.