Roshini Arivazhagan

5 Questions 10 Answers 0 Followers

Questions related from Roshini Arivazhagan

Can you offer advice on cloning microRNA in expression vector?

Hi !! Has anyone tried cloning a microRNA in expression plasmid ? i am trying to clone the premature sequence of the microRNA of interest in pCDNA3.1. i use genomic dna as template. i am not able...

07 July 2015 2,848 3 View

What is the maximum microRNA concentration that can be used for in-vitro studies?

Hi !! I would like to know what is the maximum microRNA concentration that can be used for in-vitro studies.. Like 100nM or 200nM ect.. Kindly suggest me regarding this. Thanks in advance..

05 May 2015 886 2 View

How do I amplify a 10 kb fragment via PCR?

Hi ! I need to clone a 10 kb fragment into a GFP vector. To start with the PCR amplification, I am not able to amplify it. The primer Tm are Fw- 52.3 , Rv- 50.8. The GC contents are 55.8% and 45%...

04 April 2015 907 16 View

Can anyone recommend a protocol for GLIOMA animal modelling ?

Hi Has anyone tried GLIOMA animal modelling ? It will be really helpful if anyone could kindly suggest me a good protocol to follow.

02 February 2015 6,169 5 View

Hi, I have two enzymes - EcoR1 and Kpn1. Both the enzyme expirations dates in 2010. Can I use these two enzymes for cloning?

Hi, I have two enzymes - EcoR1 and Kpn1. Both the enzyme expirations dates in 2010. Can I use these two enzymes for cloning? Will it work and, if yes, will there be proper restriction overhangs?...

10 October 2014 1,590 14 View