Md Mahmodul Hasan Sohel

7 Questions 27 Answers 0 Followers

Questions related from Md Mahmodul Hasan Sohel

Which types of collagenase is suitable for endometrial tissue digestion?

Dear all, I am trying to isolate and culture bovine endometrial stromal cells (stromal fibroblast cells) by using collagenase type II for digestion (conc. 2mg/ml). I tried tow times but the result...

04 April 2016 9,842 11 View

What are the challenges you faced during working with extracellular miRNAs?

Dear friends and fellows, It will be great to know your experiences working with extracellular/circulating or exosomal miRNAs. We are facing different problems starting from sample isolation to...

02 February 2016 9,239 2 View

Do anyone have the plate layout of miScript miRNA PCR Array Cow miRBase Profiler Plate 9 (MIBT-659ZF-24)?

Dear all, I am currently using Qiagen miRNA profiler plates (catalogue number: MIBT-659ZF-24) to identify deregulated miRNAs in cattle under certain physiological condition. I have ordered the...

12 December 2015 3,553 5 View

Is it possible to develop a cell line from granulosa cells through passaging?

Dear all, I am working with granulosa cells and I would like to know- 1. Is it possible to develop a cell line using granulosa cells? 2. If possible how many passage it require?

11 November 2015 1,467 4 View

Can anyone suggest a RBC lysis buffer recipe?

Dear all, I am working with bovine granulosa cells. Recently, I have moved to a new lab and I lost my previous protocol to prepare RBC lysis buffer which is suitable for granulosa cells collected...

02 February 2015 5,025 10 View

Is there any method to estimate miRNA concentration from raw ct values?

I am trying to to interpret miRNA expression in certain sample. I have only raw ct value. It would be very nice to include concentration in relation to ct value.. has anyone done it??? help and...

03 March 2014 3,580 3 View

Is global normalization best or any other methods for extra-cellular miRNA qPCR array data analysis?

11 November 2013 8,776 0 View