What is the best website or software by which I could determine transcription factor(s) that potentially bind to upstream my gene of interest?

I am trying to find a transcription factor(s) that potentially binds to a specific segment of my gene of interest. The segment is a little bit large (about 3kbp). knowing which transcription...

09 October 2019 2,957 2 View

What does it mean when you have less cells# in flow cytometry plot compared to a control group?

Hi! I have run flow cytometry for 3 different cell lines (black, blue, and red) which were stained with red dye (FL2) with a same event number (15,000 events). However, the cell number (count; Y...

09 October 2017 2,961 3 View

Is there any difference regard to a cell behavior after knock out a gene using different plasmids ?

01 February 2017 1,324 3 View

Is there any ideas why the over expressed protein behaves differently than the endogenous protein?

09 October 2016 916 9 View

What is the best way to test several genes expressions using qPCR?

06 July 2016 5,179 3 View

What is a good or official program to quantify western blotting results?

What is a good or official program to quantify western blotting results? Any help would be appreciated and thanks

08 September 2015 1,600 2 View

Does someone know a web sit(s) by which we can check the biological conferences in the USA?

03 April 2015 909 0 View

Is there any way to avoid cleavage of eukaryote protein expressed in E coli?

Hello, I am trying to express eukaryote protein (36 KDa) in E coli ( BL21 and Rosetta) by using different vectors (pGEX-4T-2 and pET-28a) and tags (GST and His- tag, respectively). However, more...

03 April 2015 7,830 8 View

How many times can we use GE Healthcare GSTrap FF, 1 ml?

31 December 2014 4,600 0 View

Is there any way to purified a protein that does not bind in either cation column nor anion column?

Hello, I express my protein with GST tag and trying to get my protein pure without the tag. Therefore, after I got GST-protein partially pure by performing affinity chromatography, I cut the tag...

31 December 2014 4,945 21 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View