Hello there...
Does someone know a web sit(s) by which we can check the biological conferences (specially not expensive ones) in the USA ? Thanks in advance.
I am trying to find a transcription factor(s) that potentially binds to a specific segment of my gene of interest. The segment is a little bit large (about 3kbp). knowing which transcription...
09 October 2019 3,099 2 View
Hi! I have run flow cytometry for 3 different cell lines (black, blue, and red) which were stained with red dye (FL2) with a same event number (15,000 events). However, the cell number (count; Y...
09 October 2017 3,123 3 View
CRISPR-Cas9 has been used to knock out a gene in breast cancer cell (MCF7) by using two different plasmids. One plasmid has a puromycin selection marker and another one has a GFP selection marker....
01 February 2017 1,460 3 View
I over-expressed HA tagged mammalian protein (27KD) in 3 different cell lines; however, I saw the over expressed protein (exogenous) behaves differently than the endogenous protein in the all cell...
09 October 2016 1,065 9 View
I would like to determine several gene expressions for 4 different samples using qPCR with SYBR Green. Is it possible to run all of them in one plate regard to primers Tm differences? Would run...
06 July 2016 5,337 3 View
What is a good or official program to quantify western blotting results? Any help would be appreciated and thanks
08 September 2015 1,737 2 View
Hello, I am trying to express eukaryote protein (36 KDa) in E coli ( BL21 and Rosetta) by using different vectors (pGEX-4T-2 and pET-28a) and tags (GST and His- tag, respectively). However, more...
03 April 2015 7,957 8 View
How many times can we use GE Healthcare GSTrap FF, 1 ml, which is an affinity chromatography column that can capture GST taged-protein ?
31 December 2014 4,735 0 View
Hello, I express my protein with GST tag and trying to get my protein pure without the tag. Therefore, after I got GST-protein partially pure by performing affinity chromatography, I cut the tag...
31 December 2014 5,137 21 View
Hello, I am trying to cleavage my protein from GST-tag by using Thrombin with its site ( L-V-P-R-G-S) which is provided by plasmid. However, I am confused because I saw after running my protein...
10 November 2014 6,349 10 View
This article is mine. It was republished by unknown person using an address (Wilson N Jones Regional Center, Sherman Texas USA. I have a patent.
10 August 2024 7,528 5 View
Hello all, I wanted to know, can I use galaxy (USA, Europe or Australia) platform for analyzing the shotgun data, and can it be used for publication purpose as well? Thanks :)
06 August 2024 6,610 4 View
Exism movements after gaining power within liberal democracies under majority rule and independent rule of law system become permanent dictatorship threats, but why this is the case is not clear...
04 August 2024 8,125 3 View
IEEE Conference template
02 August 2024 8,762 2 View
Since 2016 Brexit, the world needed to change the thinking behind traditional democracy as the democratic landscape changed, yet traditional democratic thinkers and actors have been acting as if...
28 July 2024 6,515 1 View
Perfect democracy thinking assumes no chaos so no need for independent rule of law system and liberal democracies assume the possibility of normal democratic chaos that can be sorted out by an...
28 July 2024 473 1 View
Do you know of any online international conferences that offer free discussions? I am looking for examples in the field of molecular ecology and DIY biology.
28 July 2024 6,501 0 View
"Integration of Mass Media and Communication into the System of International Relations: Place, Role, Actuality and Prospects: Modern Media Issues 2024" Website: https://immcir.alnoor.edu.iq/
23 July 2024 3,139 6 View
The County health department is providing Breast cancer incident data. If colorectal cancer cases could be used as control, then we can get respective data from health department as well.
22 July 2024 9,954 4 View
Their is new research published in the biological control journal, and I am one of the authors. I want to add it to my account at researchgare. How can I do this?
21 July 2024 7,545 0 View