What is a good or official program to quantify western blotting results?

More Ahmed Dhamad's questions See All

What is the best website or software by which I could determine transcription factor(s) that potentially bind to upstream my gene of interest?

I am trying to find a transcription factor(s) that potentially binds to a specific segment of my gene of interest. The segment is a little bit large (about 3kbp). knowing which transcription...

09 October 2019 3,099 2 View

What does it mean when you have less cells# in flow cytometry plot compared to a control group?

Hi! I have run flow cytometry for 3 different cell lines (black, blue, and red) which were stained with red dye (FL2) with a same event number (15,000 events). However, the cell number (count; Y...

09 October 2017 3,123 3 View

Is there any difference regard to a cell behavior after knock out a gene using different plasmids ?

CRISPR-Cas9 has been used to knock out a gene in breast cancer cell (MCF7) by using two different plasmids. One plasmid has a puromycin selection marker and another one has a GFP selection marker....

01 February 2017 1,460 3 View

Is there any ideas why the over expressed protein behaves differently than the endogenous protein?

I over-expressed HA tagged mammalian protein (27KD) in 3 different cell lines; however, I saw the over expressed protein (exogenous) behaves differently than the endogenous protein in the all cell...

09 October 2016 1,065 9 View

What is the best way to test several genes expressions using qPCR?

I would like to determine several gene expressions for 4 different samples using qPCR with SYBR Green. Is it possible to run all of them in one plate regard to primers Tm differences? Would run...

06 July 2016 5,337 3 View

Is there any way to avoid cleavage of eukaryote protein expressed in E coli?

Hello, I am trying to express eukaryote protein (36 KDa) in E coli ( BL21 and Rosetta) by using different vectors (pGEX-4T-2 and pET-28a) and tags (GST and His- tag, respectively). However, more...

03 April 2015 7,957 8 View

Does someone know a web sit(s) by which we can check the biological conferences in the USA?

Hello there... Does someone know a web sit(s) by which we can check the biological conferences (specially not expensive ones) in the USA ? Thanks in advance.

03 April 2015 1,052 0 View

How many times can we use GE Healthcare GSTrap FF, 1 ml?

How many times can we use GE Healthcare GSTrap FF, 1 ml, which is an affinity chromatography column that can capture GST taged-protein ?

31 December 2014 4,735 0 View

Is there any way to purified a protein that does not bind in either cation column nor anion column?

Hello, I express my protein with GST tag and trying to get my protein pure without the tag. Therefore, after I got GST-protein partially pure by performing affinity chromatography, I cut the tag...

31 December 2014 5,137 21 View

Which are the amino-sequence cutting sites of bovine thrombin?

Hello, I am trying to cleavage my protein from GST-tag by using Thrombin with its site ( L-V-P-R-G-S) which is provided by plasmid. However, I am confused because I saw after running my protein...

10 November 2014 6,349 10 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

How to solve g_mmpbsa error?

Program: g_mmpbsa, version 2024.1 Source file: extrn_apbs.cxx (line 152) Fatal error: Failed to execute command: $APBS pybYcUWA.in --output-file=pybYcUW.out

07 August 2024 6,066 0 View

Are students in the MBA program in Fisheries Enterprises Management eligible to work in the aquaculture industry abroad?

Are students in the MBA program in Fisheries Enterprises Management eligible to work in the aquaculture industry abroad? Could you explain the foreign countries interested in recruiting...

28 July 2024 8,378 2 View

How can we calculate the percentage of configuration interaction (CI) in the UV output data of the Gaussian program?

How can we calculate the percentage of configuration interaction (CI) in the UV output data of the Gaussian program? for example: Excited State 17: Singlet-A 5.1359 eV 241.41 nm...

28 July 2024 9,165 2 View

Someone have the key or the installer of the program Mx pro 3000 (PCR real time)?

I need to install this program

25 July 2024 4,756 0 View

Has the Affordable Connectivity Program Helped You/Your Community? And Would Bulk Tablets on a Similar Program Help Your Business?

Do You Believe VP Harris Would Show Empathy to Tech Companies in Her Administration as POTUS? I ask in light of the fact that, the ACP was not re-funded this round. So many and businesses need...

24 July 2024 4,732 3 View

How to quantify the fluorescence intensity of only the merge area of an image using ImageJ software?

Dear All: I have to quantify the fluorescence intensity of cells that are fluorescent to two different markers, so i'm interested in finding out if this is possible. Previously I have...

22 July 2024 8,770 1 View

What will be the best way to test NFkb activation via western blot?

Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?

18 July 2024 5,214 4 View

Please, from the computational calculations of an organic compounds using Gaussian program?

Using DFT/B3LYP/6-311++G

17 July 2024 7,720 1 View

How to detect phosphorylation on Western Blots?

Having trouble detecting phosphorylated HSL. Any tips for how to prevent de-phosphorylation events or detect phosphorylation?

15 July 2024 2,055 3 View