Ahmed Dhamad

23 Questions 193 Answers 0 Followers

Questions related from Ahmed Dhamad

What is the best website or software by which I could determine transcription factor(s) that potentially bind to upstream my gene of interest?

I am trying to find a transcription factor(s) that potentially binds to a specific segment of my gene of interest. The segment is a little bit large (about 3kbp). knowing which transcription...

10 October 2019 3,078 2 View

What does it mean when you have less cells# in flow cytometry plot compared to a control group?

Hi! I have run flow cytometry for 3 different cell lines (black, blue, and red) which were stained with red dye (FL2) with a same event number (15,000 events). However, the cell number (count; Y...

10 October 2017 3,090 3 View

Is there any difference regard to a cell behavior after knock out a gene using different plasmids ?

CRISPR-Cas9 has been used to knock out a gene in breast cancer cell (MCF7) by using two different plasmids. One plasmid has a puromycin selection marker and another one has a GFP selection marker....

02 February 2017 1,427 3 View

Is there any ideas why the over expressed protein behaves differently than the endogenous protein?

I over-expressed HA tagged mammalian protein (27KD) in 3 different cell lines; however, I saw the over expressed protein (exogenous) behaves differently than the endogenous protein in the all cell...

10 October 2016 1,041 9 View

What is the best way to test several genes expressions using qPCR?

I would like to determine several gene expressions for 4 different samples using qPCR with SYBR Green. Is it possible to run all of them in one plate regard to primers Tm differences? Would run...

07 July 2016 5,310 3 View

What is a good or official program to quantify western blotting results?

What is a good or official program to quantify western blotting results? Any help would be appreciated and thanks

09 September 2015 1,706 2 View

Does someone know a web sit(s) by which we can check the biological conferences in the USA?

Hello there... Does someone know a web sit(s) by which we can check the biological conferences (specially not expensive ones) in the USA ? Thanks in advance.

04 April 2015 1,020 0 View

Is there any way to avoid cleavage of eukaryote protein expressed in E coli?

Hello, I am trying to express eukaryote protein (36 KDa) in E coli ( BL21 and Rosetta) by using different vectors (pGEX-4T-2 and pET-28a) and tags (GST and His- tag, respectively). However, more...

04 April 2015 7,932 8 View

How many times can we use GE Healthcare GSTrap FF, 1 ml?

How many times can we use GE Healthcare GSTrap FF, 1 ml, which is an affinity chromatography column that can capture GST taged-protein ?

01 January 2015 4,710 0 View

Is there any way to purified a protein that does not bind in either cation column nor anion column?

Hello, I express my protein with GST tag and trying to get my protein pure without the tag. Therefore, after I got GST-protein partially pure by performing affinity chromatography, I cut the tag...

01 January 2015 5,101 21 View

Which are the amino-sequence cutting sites of bovine thrombin?

Hello, I am trying to cleavage my protein from GST-tag by using Thrombin with its site ( L-V-P-R-G-S) which is provided by plasmid. However, I am confused because I saw after running my protein...

11 November 2014 6,322 10 View

Can I separate two proteins which have almost the same M.Wt. and isoelectric points of 7.1 and 7.75 using IEC?

I have two proteins which have almost the same M.Wt. They also have isoelectric points (7.1 and 7.75). Can I separate them with Ion exchange chromatography(IEC)? What is the PI range by which Ion...

10 October 2014 10,016 7 View

What is the best approach to identify the cofactors (co-activators & co- repressors) that bind protein x, while protein x is binding histones?

Hello, I know for sure protein x binds and modifies histones and am looking for identifying its cofactors that potentially involve in the binding and modifications. what is the best way to reach...

09 September 2014 9,504 4 View

Does anyone perform S-Carboxymethylation of Cysteine for protein or peptide? I am looking for the protocol . Any help would be appreciated

I am looking for the S-Carboxymethylation of Cysteine protocol. I want to methylate my protein by using iodoacetic acid. If you have a procedure for that, please send my a copy or tell me where I...

09 September 2014 328 5 View

What is the best or common approach by which we can detect co-factors (activators and repressors) of a nuclear receptor in the nucleus?

What is the best or common approach by which we can detect co-factors (activators and repressors) of a nuclear receptor (e.g. androgen receptor) in the nucleus of a cancer cell? Any help would...

08 August 2014 7,757 4 View

Is there any comprehensive website or tool to investigate the protein protein interactions?

I am looking for a website or tool by which I can see in broad range the interactions between two mammalian proteins.

08 August 2014 7,447 8 View

Is the carboxymethylation of a protein important for crystallization and what is the best approach to carboxymethylate a protein?

I am working on a protein fragment and eventually I am going to crystallize it with another protein. I read one paper about the crystallization of that fragment and it said the carboxymethylation...

08 August 2014 3,044 4 View

What is the main function(s) of Heat Shock Proteins (HSPs) in the nucleus and cytoplasm?

I am working on Heat shock proteins ( HSC70, HSP70, and HSP90) and have seen them migrate from cytoplasm to the nucleus or vise versa in the cancer cell line (MCF7). Is there any explanation for...

07 July 2014 9,509 4 View

Is it important to know how many passages of the cell line have passed?

I am working with MCF7 and 293T cells. Do I have to know the # of passages that they have passed? If yes, what is the maximum passage's number, in general to my cells?

05 May 2014 3,236 18 View

How can I get rid of a non-specific binding with GST- beads?

I am doing protein mapping to my interesting protein (chop it off for different domains, mix them with other GST- protein to do in vitro binding assay and then they are pulled down with GST-...

05 May 2014 7,735 8 View

What are the limitations of using MS (mass spectrometry) in terms of studying protein- protein interactions?

See above.

05 May 2014 572 4 View

Does anyone know how to get more than 50 nucleotides upstream and downstream in a coding gene sequence?

I have the DNA coding sequence to my intrest protein, but I want to get at least 50 nucleotides up and downstream. Is there any website or program to get that?

03 March 2014 6,514 5 View

Does anyone know how to get the 3D structure of whole Human Estrogen Receptor alpha (ERa)?

I am working on Human Estrogen Receptor alpha (ERa). I really need the 3D structure of ERa even predict one. I have the DNA and amino acid sequence of this protein, and I have tried some websites,...

12 December 2013 6,654 11 View