02 February 2017 3 1K Report

CRISPR-Cas9 has been used to knock out a gene in breast cancer cell (MCF7) by using two different plasmids. One plasmid has a puromycin selection marker and another one has a GFP selection marker. Although we use the same gRNA to knock out the gene, we have seen different cell's behavior after the gene was knocked out. Is there any idea why the difference? Does puromycin/ GFP markers affect the cells? I appreciate any help.

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