Housekeeping protein for mice myoblast cell lines
I have had good results in all muscle tissue with B-Tubulin.
Thank you Chad.
I think you should also consider actin and GAPDH.
Can we find Urbach energy from the Tauc's plot itself? That means, by extrapolating the urbach tail to x axis of Tauc's plot..
26 July 2023 423 0 View
Which can be ideal RNA markers for cell fractionation, as in for nuclear fraction and cytoplasmic one? I need to work on lncRNA localization in the different cell fractions. Thanks in Advance!!!
28 February 2022 6,846 1 View
Hi, I am trying to use a cytoplasmic nuclear fractionation kit which is employed for protein analysis. However, I need to analyze the RNA as well using the kit and was looking for any RNase...
21 February 2022 5,760 1 View
willing to publish a paper
03 June 2019 5,607 2 View
Why the output voltage of inverter is zero eventhough the pulses are coming correctly from dSPACE controller.
24 April 2018 4,792 5 View
The protocols available are optimised at ph 6.8 to 7.
07 September 2017 8,939 2 View
I have done F- EMSA and used 200nm of my DNA and titrated it with different concentration of my repressor protein. I found the kd to be 1.6 micro molar and I used prism sofware to fit my data with...
24 September 2015 5,477 3 View
Hi fellow researchers, am an newbie in protein purification and hence this question. I am working on a novel protein whose size is 17kda.it does not have cysteine. It's PI is around 7.83. I did a...
20 June 2015 2,793 5 View
Why do researchers serum starve the cells before studying the molecular event in a cell line. I am planning to look at the Insulin signalling events in skeletal muscle cell lines, should I serum...
27 February 2015 7,115 5 View
Do anyone have an idea about PEDF secretion from skeletal muscle.
11 February 2015 7,695 1 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
