Housekeeping protein for mice myoblast cell lines
I have had good results in all muscle tissue with B-Tubulin.
Thank you Chad.
I think you should also consider actin and GAPDH.
willing to publish a paper
03 June 2019 5,402 2 View
The protocols available are optimised at ph 6.8 to 7.
07 September 2017 8,796 2 View
I have done F- EMSA and used 200nm of my DNA and titrated it with different concentration of my repressor protein. I found the kd to be 1.6 micro molar and I used prism sofware to fit my data with...
24 September 2015 5,366 3 View
I have run western blots and have got clean bands for AKT, phospho Akts and others but I am not able to get clear bands on repeating the western from the same samples around 3 weeks later. My...
30 December 2014 4,990 5 View
I have done my western blotting using C2C12 cell lysate sample but I am not able to get the band for the above. I am using Santa Cruz antibodies for the insulin receptor beta subunit and its...
14 December 2014 8,237 4 View
I have used commercially control and target siRNA from SantaCruz to perform transfection in C2C12 and HSMM. I performed western blot and the target protein was not expressed while the controls...
24 November 2014 1,757 15 View
i am using a polyclonal rabbit primary for detecting a 50KDa protein but keep getting nonspecific band around 30KDa and few others. I block using 5% non fat milk for one hour sample preparation by...
28 October 2014 3,198 7 View
28 September 2014 8,007 5 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...
03 March 2021 8,796 3 View
I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...
03 March 2021 4,116 4 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
