Chad D. Touchberry

4 Questions 25 Answers 0 Followers

Questions related from Chad D. Touchberry

How do I arrest the heart in diastole prior to cardiac histology?

I need to arrest the heart in diastole prior to fixation to analyze cardiomyocyte size using WGA. I have seen publications using 40 mM KCl, but I have concerns that such a hypotonic solution will...

14 August 2015 2,028 2 View

How do I ship RNA from primary mouse adult ventricular cardiomyocytes?

I am treating cultured AVCMs from mice and analyzing gene expression with a collaborator. Normally, I would just isolate the total RNA from the culture wells and perform PCR. In this specific...

28 April 2015 6,381 2 View

Has anyone made or purchased their own Parallel-Plate Flow Chamber or Shear Stress device?

I am looking for a cost-effective way to make or purchase a shear stress device to provide fluid shear stress to cells in monolayer culture (0-10 dynes). I received a quote from Flexcell for the...

15 July 2014 8,280 7 View

Can anyone help with patch clamping of adult mouse cardiomyocytes?

We are unable to patch adult ventricular cardiomyocytes from mice. Our initial seal is terrible and leaky. Ca++ floods in and the cell begins to beat. We get ~ 1 million nice rod-shaped, striated...

14 November 2013 9,420 2 View