What is the half-life of Withaferin A in culture? By what process is it broken down?

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Can you process tissue for FFPE previously cryoprotected in sucrose?

If tissue was fixed in 4% PFA then cryoprotected in 30% sucrose, are you able to wash and switch to FFPE processing instead of blocking frozen in OCT?

03 October 2023 198 3 View

Differnce between using human vs. mouse FLT3L when growing BMDCs?

Are there phenotypic and functional differences in DCs generated from culturing BMDCs (mouse) in the presence of GM-CSF vs. FLT3L. Also does using human vs. mouse FLT3L make a difference? Many...

03 August 2023 877 1 View

Any tips for RNA extraction from stool matrix using RNeasy Mini Kit?

Product of interest: ZeptoMetrix Norovirus GI and GII External Run Controls. https://www.fishersci.com/shop/products/nattrol-gp-external-run-controls/21100555 I am trying to run the RNA...

07 May 2023 3,968 3 View

How do I prevent plasma from turning jelly-like when stored at 4 degrees celsius?

I have a mixture of goat serum / plasma that I attempted to ammonium sulfate precipitate (using pooled samples) to get out the immunoglobulins. I dialyzed in PBS afterwards, but when placed in the...

29 March 2023 3,791 1 View

Using both conjugated and unconjugated antibodies in multiplex fluorescent Western Blot?

Is it possible to obtain readable results using a loading control conjugated antibody (e.g., Rb anti-Gapdh 680) along with a target protein unconjugated antibody plus secondary, if the target...

14 March 2023 2,821 2 View

Every time i turn the air pump on bubbles start to come out... what should i do?

I am trying to grow spirulina at home but i am a bit confuse. Now every time I turn the air pump on the water starts to create some bubbles so I can't turn it on because it will spill all of my...

17 February 2023 5,461 1 View

How does online video games socially impact an individual?

Finding the links between behavior and online multiplayer gaming.

03 February 2023 4,727 0 View

Creating a Sample Dilution to Load for Gel Electrophoresis?

Hello, I am currently working with serum samples to perform a Western Blot to identify a series of target proteins. I am having trouble calculating the amount of sample I need to load. I would...

28 January 2023 9,654 1 View

How could you extract mycelium from the soil?

I am working on a mycoremediation project and trying to sperate the mycelium from the soil it is growing in to test for pollutants. Just looking for some new ideas.

17 January 2023 6,876 2 View

Is there an accepted, NON DESTRUCTIVE protocol for surveying insect diversity and abundance? An accepted length of time to observe an area of flowers?

as an educator in conservation, it is very important that i teach methods that are non destructive. such as counting insect visitation to flowers, for pollinator diversity and abundance studies. i...

07 December 2022 4,003 5 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

How do I conformally do PR spin coating on trench structure?

I did PR spin coating on trench structure. I used AZ P4620 PR and the thickness or PR is around 11um. The substrate is Si. And my trench structure depth is 141um(negative way). Even though I...

08 August 2024 2,298 0 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View