Finding the links between behavior and online multiplayer gaming.
If tissue was fixed in 4% PFA then cryoprotected in 30% sucrose, are you able to wash and switch to FFPE processing instead of blocking frozen in OCT?
03 October 2023 198 3 View
Are there phenotypic and functional differences in DCs generated from culturing BMDCs (mouse) in the presence of GM-CSF vs. FLT3L. Also does using human vs. mouse FLT3L make a difference? Many...
03 August 2023 877 1 View
Product of interest: ZeptoMetrix Norovirus GI and GII External Run Controls. https://www.fishersci.com/shop/products/nattrol-gp-external-run-controls/21100555 I am trying to run the RNA...
07 May 2023 3,968 3 View
I have a mixture of goat serum / plasma that I attempted to ammonium sulfate precipitate (using pooled samples) to get out the immunoglobulins. I dialyzed in PBS afterwards, but when placed in the...
29 March 2023 3,791 1 View
Is it possible to obtain readable results using a loading control conjugated antibody (e.g., Rb anti-Gapdh 680) along with a target protein unconjugated antibody plus secondary, if the target...
14 March 2023 2,821 2 View
I am trying to grow spirulina at home but i am a bit confuse. Now every time I turn the air pump on the water starts to create some bubbles so I can't turn it on because it will spill all of my...
17 February 2023 5,461 1 View
Hello, I am currently working with serum samples to perform a Western Blot to identify a series of target proteins. I am having trouble calculating the amount of sample I need to load. I would...
28 January 2023 9,654 1 View
I am working on a mycoremediation project and trying to sperate the mycelium from the soil it is growing in to test for pollutants. Just looking for some new ideas.
17 January 2023 6,876 2 View
as an educator in conservation, it is very important that i teach methods that are non destructive. such as counting insect visitation to flowers, for pollinator diversity and abundance studies. i...
07 December 2022 4,003 5 View
I was wondering what questions are best to ask participants when measuring Leadership and its effectiveness or relationship with a certain variable like hotel performance? #Leadership...
17 November 2022 9,396 13 View
Exism movements after gaining power within liberal democracies under majority rule and independent rule of law system become permanent dictatorship threats, but why this is the case is not clear...
04 August 2024 8,125 3 View
How Social Media Affects Your Mental Health
04 August 2024 6,961 3 View
On a personal note, even though technology has attracted a lot of interest and funding to combat climate change, it is becoming more and more clear that taking care of the "Human Dimension" is...
02 August 2024 6,773 12 View
I am currently researching the impact of environmental toxins on children's health and would greatly appreciate insights from experts in the field. If you are an expert or researcher working on...
02 August 2024 4,474 2 View
Hello, Dear friends, I am surveying as part of my thesis research and would greatly appreciate your participation. The study investigates how social media influences dental health behaviors...
01 August 2024 9,349 2 View
AI-driven technologies offer previously unheard-of capabilities to process enormous volumes of data, extract insightful knowledge and improve predictive models, according to the UN’s World...
31 July 2024 9,733 3 View
Perfect democracy thinking assumes no chaos so no need for independent rule of law system and liberal democracies assume the possibility of normal democratic chaos that can be sorted out by an...
28 July 2024 473 1 View
I have observed in some cases there are large negative values of surface geopotential in Era5 and MERRA2, in most cases in regions with complex orography. Why this happens? How can i handle this?
27 July 2024 4,628 2 View
The Likert scale is a commonly used measurement tool in social science research to assess people's attitudes, opinions, or perceptions. The social science students still have difficulty selecting...
25 July 2024 7,535 1 View
I am starting to work on this question, with particular reference to what we might be able to learn from countries outside the UK. Of course this topic is huge, and I am breaking sustainability...
25 July 2024 1,545 3 View