Cássio Prinholato da Silva

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Questions related from Cássio Prinholato da Silva

What is the good stanining/protocol?

I want to stain my bacterial cells to highlight the nucleus on the surface where my protein is attached. I have tried using Hoechst, but the signal in flow cytometry was too weak, resulting in a...

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Where I can find The African Spiny Mouse (Acomys cahirinus)?

Does anyone know where I can find the African spiny mouse (Acomys cahirinus)? Is there a laboratory to buy it from? Or does anyone know someone I can contact to ask about them? Regards,

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What are the possible problems why I can't see my protein express in C3030 bacteria with p28a (+)?

I have been trying to express a protein, but I won't get success. My conditions for expression were: C3030 bacteria LB media + kan (50ug/mL) O.D = 0.4-0.5 IPTG 0.4mM -0.5mM 37C for 4 hours or 16C...

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