What are the possible problems why I can't see my protein express in C3030 bacteria with p28a (+)?

I have been trying to express a protein, but I won't get success. My conditions for expression were: C3030 bacteria LB media + kan (50ug/mL) O.D = 0.4-0.5 IPTG 0.4mM -0.5mM 37C for 4 hours or 16C...

10 February 2023 9,770 3 View