Arbitrary cut-off values for forest using NDVI?

I'm trying to use NDVI data to define forested land in the southeastern United States. The data is made up of 1m pixels. My goal was to classify each pixel as vegetation or not, then use a cut-off...

22 May 2019 1,251 8 View

Why is my media turning black?

I have a cell line that causes DMEM to turn a dark purple/black colour when the cells are 100% confluent. However, this only happens when the cells are stably transfected with a specific plasmid,...

17 March 2019 3,621 4 View

Literature on not providing care as not wanting to be accountable/take resposibility?

Hi, I'm writing an essay currently on an ethical dilemma were someone i cared for was catatonic. I'm looking for literature aound healthcare professionals not wanting to provide certain types of...

16 February 2018 6,503 3 View

Antibodies for targeting CRF1 and CRF2?

Does anyone known of any antibodies I can use to tag CRF1 and CRF2 receptors?

23 July 2017 8,862 1 View

QPCR Cost Analysis

I'm doing a cost analysis for a PCR experiment and want to make sure I have left anything out that I may need. The list is currently as follows: Primers, TRIzol Reagent, RNAse Free Water, RNAse...

09 July 2017 4,830 4 View

Does anyone know of a good housekeeping gene for gfap and/or Glt1 expression?

In need of a reliable housekeeping gene to normalize my pcr data to. Targeting Gfap and GLT-1 genes.

02 July 2017 567 4 View

How much tissue can I expect to get from a VTA dissection of mice brain?

I'm trying to anticipate how many mice I'll need for RT-qPCR of several genes expressed in the midbrain, specifically the VTA. I need to be able to get about 4mg of VTA tissue from each brain. Is...

29 June 2017 3,703 3 View

What RNA isolation kit would you recommend I use in isolating RNA from brain tissue?

I'm doing RT-qPCR and was wondering which RNA isolation kit I should use.

29 June 2017 8,327 3 View

How do you perform protein/antibody viscosity measurements?

I was wondering for those out there how you currently perform protein viscosity measurements (or antibodies)? What do you use the viscosity measurements for/why do you care about the viscosity?...

27 November 2016 7,490 4 View

Does anyone know what measure of level of parental supervision was used in the Great Smoky Mountain Study?

25 February 2015 3,987 1 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

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02 March 2021 3,060 3 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View