Arbitrary cut-off values for forest using NDVI?

I'm trying to use NDVI data to define forested land in the southeastern United States. The data is made up of 1m pixels. My goal was to classify each pixel as vegetation or not, then use a cut-off...

22 May 2019 1,251 8 View

Why is my media turning black?

I have a cell line that causes DMEM to turn a dark purple/black colour when the cells are 100% confluent. However, this only happens when the cells are stably transfected with a specific plasmid,...

17 March 2019 3,621 4 View

Literature on not providing care as not wanting to be accountable/take resposibility?

Hi, I'm writing an essay currently on an ethical dilemma were someone i cared for was catatonic. I'm looking for literature aound healthcare professionals not wanting to provide certain types of...

16 February 2018 6,503 3 View

Antibodies for targeting CRF1 and CRF2?

Does anyone known of any antibodies I can use to tag CRF1 and CRF2 receptors?

23 July 2017 8,862 1 View

QPCR Cost Analysis

I'm doing a cost analysis for a PCR experiment and want to make sure I have left anything out that I may need. The list is currently as follows: Primers, TRIzol Reagent, RNAse Free Water, RNAse...

09 July 2017 4,830 4 View

Does anyone know of a good housekeeping gene for gfap and/or Glt1 expression?

In need of a reliable housekeeping gene to normalize my pcr data to. Targeting Gfap and GLT-1 genes.

02 July 2017 567 4 View

How much tissue can I expect to get from a VTA dissection of mice brain?

I'm trying to anticipate how many mice I'll need for RT-qPCR of several genes expressed in the midbrain, specifically the VTA. I need to be able to get about 4mg of VTA tissue from each brain. Is...

29 June 2017 3,703 3 View

What RNA isolation kit would you recommend I use in isolating RNA from brain tissue?

I'm doing RT-qPCR and was wondering which RNA isolation kit I should use.

29 June 2017 8,327 3 View

How do you perform protein/antibody viscosity measurements?

I was wondering for those out there how you currently perform protein viscosity measurements (or antibodies)? What do you use the viscosity measurements for/why do you care about the viscosity?...

27 November 2016 7,490 4 View

What is the fundamental use of Sorensen's glycine buffer?

18 May 2015 6,877 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Genescan gives 3 peaks instead of one?

Hi all, I am doing a genescan analysis for a deleted exon in the patient sample. The amplified region is 215 bp. In the patient sample because of homozygous deletion, no peak is observed while in...

23 February 2021 5,645 1 View

How to find if Qualitative and Quantitative (discrete) variables have a relation?

I'm going to do a brief research about the dependance between the number of cases of Covid-19 and sex( male/female) . One of the requierements is to use inferential statistics to test the...

18 February 2021 7,092 11 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

Can Immunogenic BNT162b vaccines protect HUMANS from SARS-CoV-2?

"A safe and effective vaccine against COVID-19 is urgently needed in quantities sufficient to immunise large populations. We report the preclinical development of two BNT162b vaccine candidates,...

11 February 2021 6,628 9 View

Is there any publication about the effect of basketball and volleyball on body height?

Many people believe that some stretching sports with vertical jump such as basketball and volleyball can increase the body height. On the other hand, others do not agree with this argument. The...

02 February 2021 281 6 View

Difference between assessing EC50 in presence of physiological levels of human serum albumin and alpha1 acid glycoprotein vs. % human serum?

01 February 2021 1,825 1 View

Why does the testosterone dissolved in dmso and methanol turns into a white dispersed precipitate when subjected to serum free media?

1mg of testosterone is dissolved in 20 microlitre of DMSO and Methanol respectively. The interpretation showed a positive result. However, when 1 ml of serum free media is subjected to the...

27 January 2021 8,213 1 View

How would you assess protein binding adjusted EC50 for a drug that has a binding rate of over 95%?

I'm in need of a reference or a method to come up with a protocol that allows one to get the protein-adjusted EC50 for an antiviral drug in presence of human serum albumin and acid glycoprotein.

27 January 2021 1,402 1 View