Immunofluroscence LC3 protocols and conditions?

Hi I am looking for an optimized protocol for Lc3 and lamp 2 immunofluroscene.currently facing a problem,,have been tried with different conditions as per few papers,but could not able to make...

06 July 2014 8,142 4 View

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Hello, Does anyone sucessed to make THP1 cell transfection efficiency? I am currently trying to tranfect THP1 adherent cells (PMA differentiated for 10 hrs) with specific vendor protocol. But it...

06 July 2014 2,515 8 View

What is the difference between co-infections and mixed infections?

Do we consider a co-infection as invitro infection of two different pathogens to a single cell type, or infection of two different pathogens to an animal model. Like wise Mixed infection-the term...

08 September 2013 6,289 10 View

Iron homeostasis and Intracellular microbial defense

As a bonafide statement, Iron is an important sustain for the colonization of microbes in the host. Targeting microbial iron acquisition system may also a crucial therapeutic way to fight against...

06 July 2013 2,686 0 View

How does an inhibitory signal cascade re-activate (how the downregulation cascade reactivates for upregulation)?

How does an inhibition complex reactivate again for its own signal transduction. I am interested to know the possibilities of a kinetic mechanism of signaling cascade of inhibitory complex. We...

04 May 2013 7,675 0 View

What is the standard method for assay co-infection experiments in vitro?

I am trying to perform a co-infection experiment on activated macrophage and I want to know discrimination of Immune regulations. What kind of experimental assaying outlines can resolve my...

01 February 2013 4,919 2 View

Research has deviations in its mode? Why we RE-search?

As a mind of philosopher-research has deviations in its mode with an initial level of thinking,formulating and hypothesis.But all hypothesis are not a well defined laws. All hypothesis will...

11 December 2011 6,808 0 View

Hi to all group members

Here I would like to ask you a question Regarding continuation of desired work on Diagnostic tool development Project title: Avian flu Neuraminidase gene expression ,purification and...

09 October 2010 9,882 0 View

Intrested on Research ,Specially expression studies of Recombinant proteins in Prokaryotic Sysems And Quantification of desired proteins by Biophysical techniques

Hi . Here Srikanth, A student of Life science having lots of Practical Ideas.. Currently looking to take a chance

09 October 2010 6,567 0 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View